Re: Rapid transplant bx processing

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From:"Bill Sinai (Anatomical Pathology)" <Bills@icpmr.wsahs.nsw.gov.au> (by way of histonet)
To:histonet@histosearch.com
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Dear All,

Since 1984 we have been using a BioRad H2500 Microwave Processor to
process  renal biopsies.

Our Protocol:

Fixation:           solution not important   10min @ 60degreesC
Dehydration:    100% alcohol                  8 min @ 60C
                        100% alcohol                  10min @ 60C
                        Isopropanol                    8 min @ 60C
                        Isopropanol                   10min @ 60C
Infiltration:       Paraplast Wax in vacuum embedder under 15mm
                        mercury @ 60c TWO CHANGES 10 MINS.

The pathologist usually has the slides in under 2 hours and reported
in under 3 hours.

Bill Sinai
Department Manager
Tissue Pathology
ICPMR Westmead Hospital
WESTMEAD NSW AUSTRALIA
Phone 61+2+9845 7774  Fax 61+2+9687 2330
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