Hi All,
Can anyone tell me if there are an differences or advantages in using the
method suggested below verus the one I heard of a shot time ago.  This
staining procedure I heard of was found in an article in The Journal of
Histotechnology Volume 16, Number 2, June of 1993. It involves staining
with Astra Blue and Vital new Red at an acid Ph in formalin fixed paraffin
embedded human tissue. These procedures may even be the same or similar I
don't know?  I have used the 1993 stain and it stains mouse eosinophils a
nice red color but not as brilliant red and as in the article using human
tissue, any thoughts on this? This 1993 stain works I just might want to
find a procedure that makes the stain a little more brilliant, any thoughts
or comments is very much appreciated.

Jamie Erickson
Associate Scientist
 Genetics Institute
Andover, MA

>>> "Tarpley, John" <jtarpley@amgen.com> 12/13 1:40 PM >>>
Gayle,
Thanks for the kind words. The method I published just allowed me to stand
on the shoulders of some of the giants of our profession by combining their
methods for mast cells and eosinophils. The reference is Tarpley JE, C.
Meshter, and D. Tyler. Combination Stain for Mast Cells and Eosinophils,  J
of Histotechnology, vol. 7, 1984.

John Tarpley 15-2-B
Associate Scientist
Specialist Image Analysis & Immunohistochemistry
Amgen Inc
One Amgen Center Drive
Thousand Oaks, CA  91320


> ----------
> From: 	Gayle Callis[SMTP:uvsgc@msu.oscs.montana.edu]
> Sent: 	Monday, December 13, 1999 9:17 AM
> To: 	histonet@pathology.swmed.edu
> Subject: 	eosinophil stain
>
> Hopefuly John Tarpley will come forth with his reference from Journal
> of Histotechnology article on eosinophil staining, it was quite elegant.
> Will try and dig it out today.
>
> Gayle Callis
>