This is essentially the same carbohydrate technique used as the final
step in demonstrating phosphorylase and branching enzyme activity in
muscles. The problem with most standard mounting techniques is rapid
fading. Two techniques often used are glycerine jelly with iodine
added or blot dry (without rinsing iodine out) and then coverslip
with a xylene or toluene based medium.

Bert

> 	----------
> 	From:  lmiller@wlgore.com [SMTP:lmiller@wlgore.com]
> 	Sent:  Tuesday, December 14, 1999 11:54 AM
> 	To:  histonet@pathology.swmed.edu
> 	Subject:  Oil of Origanum
>
>
>
> 	Hi all!
> 	I've been asked to do an iodine stain for carbohydrates, and the one
> listed in
> 	Sheehan's calls for mounting in oil of origanum. Is there something
> that I can
> 	use instead?
>
> 	Thanks in advance,
> 	Lori Miller
>
>
>

----------------------
Bert Dotson
amdj@duke.edu