Re: Ki-67

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From:Dana Settembre <settembr@UMDNJ.EDU> (by way of histonet)
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On Thu, 2 Dec 1999 wrote:

> Where do people buy their Ki-67?  I purchased a monoclonal that is supposed
> to work on frozens as well as a polyclonal from DAKO and both were awful.
> Andrea T. Hooper
I use Dako's Ki-67 on paraffin embedded tissue.  The cat# A0047 product
that I use is at a 1:50 dilution using a 30 minute citra microwave
pretreatment.  I use their LSAB2 kit for visualizing and have used other
kits as well.  They all work fine.  Maybe the problem lies in the
pretreatment.  Good Luck.

Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey

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