Re: Gallyas stain for Alzheimers

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From:"LuAnn Anderson" <> (by way of histonet)
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Responding to the message of <>
from pam pyle <>:

Hi Pam,
We tried this method several years ago and our Neuropathologists were not
with it.  While plaques were visible, neurofribillary tangles did not stain
well.  They prefer our Bielschowsky's method,(perhaps out of habit) and we
worked it out to be extremely consistant and reliable (beware of humidity!!).
Just my two cents.  Happy Holidays to all! LuAnn
> Carol,
> If you want to try a CHEAPER and FASTER silver stain for the plaques and
> tangles in Alzheimers, do I have a stain for you!!!
> I found the procedure in the Journal of Histotechnology several years
> ago, tried it, the docs loved it and we never looked back!  It uses and
> was(I think) labeled with the word(s) Methenamine Silver & Plaques &
> Tangles.  I apologize for no article specifics, but I know there are
> listeners out there who will be able to find it quickly.  I have just
> recently retired and moved to a new city.  Unfortunately that
> information didn't come with me!  I will give you a run down on the
> procedure from memory.  I urge you to give the procedure a try-it is
> truly cheaper and faster!!
> Methenamine Silver for Plaques and Tangles
> Use the same methenamine silver that is used for the GMS.
> Place 60mls(or more if you have too much boiling away) silver solution
> into a plastic coplin jar with a lid into a dish of distilled water.
> Place all of this into a microwave (ours was/is 1000W).
> If the solution is refrigerator temperature, microwave at full power for
> 1minute.  Swirl the coplin jar to distribute the heat. Wear gloves
> Microwave for another 30-45 seconds, swirl again.
> Check microscopically for darkness of plaque staining.  I find it
> difficult to identify tangles at this step, but plaques in the control
> are easy to find.  Continue microwaving in  30 second(or more or less,
> depending on your microwave) increments until the plaques are black.
> Rinse slides in distilled water.
> Place in sodium thiosulfate (2% to 5%) for 5 minutes.
> Rinse again.
> Dehydrate, coverslip. Voila!!
> Good luck!!
> Pam Pyle
> formerly of University of Texas M. D. Anderson Cancer Center, Dept of
> Pathology, Section of Neuropathology/Hybridization Lab
> .

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