Re: Epidermis artifact
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From: | "Beckers" <msadk@worldnet.att.net> (by way of histonet) |
To: | histonet@histosearch.com |
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Content-Type: | text/plain; charset="us-ascii" |
Question to everyone out there-has anyone had the problem of epidermal
cells coming out clear and or hardly stained at all after H&E or Toluidine
Blue staining? The whole section is faint. I think the tissue has dried
before fixation. I cut frozens. At times after I have finished putting 2
or 3 frozen sections on the glass slide I notice the epidermal ridge has
whitened and appears drier that the rest of the section. Just an idea. I
am trying to fix them immediately now by picking up 2 or 3 sections at once
in the cryostat. I line them up on space on the face plate and mount them
all at once. I choose not to use a anti-roll plate. This does not always
happen but at times from one slide I am lucky to have one properly stained
skin piece while the rest is faded or not stained at all. I use Rapid Fixx
for 15 seconds for H&E and 2 cups of 200 proof alcohol 1 min each for Tol.
Blue stains. I have changed all solutions and that does not seem to help.
Thanks in advance. :)
Sue Becker
Dr. MJ Mulvaney
Private practice
Albnay, NY 12205
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