On Wed, 1 Dec 1999, Trivett Melanie (PMC) wrote:

> Dear Histonetters,
>
> I am keen to hear of success stories for immunohistochemistry of cyclin D1
> and CD5 on formalin fixed sections.  Anyone willing to share protocols,
> choice of primary antibodies?  Thanks in anticipation.
>
> Melanie.
>
>
> Melanie Trivett, PhD
> Department of Pathology
> Peter MacCallum Cancer Institute,
> East Melbourne, Victoria, Australia.
>
>
>
>
>
>
Hello Melanie from Australia

I use CD5 from NovoCastra Laboratories cat# NCL-CD5-4C7.  I pretreat with
a citra buffer for 15 minutes in the microwave. I use the antibody at a
dilution of 1:25 on my Dako autostainer along with Dako's LSAB2 kit. Other
secondary and tertiary kits work fine too.  Your dilution may vary of
course. I use tonsil for a positive control. Works nicely. Good Luck.

Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey
USA