Sorry. I forgot to say what the processor is.

It is the new Ventana Renaissance, formerly MVP. It has vacuum and pressure
available during processing.

> -----Original Message-----
> From:	Andrea Grantham [SMTP:algranth@u.arizona.edu]
> Sent:	December 14, 1999 3:25 PM
> To:	Terrett, Barb
> Subject:	Re: processing mouse organs
>
> What is the processor that you have acquired? Is it one of the fancy
> enclosed vacuum processors or one of the older dipper/dunker types?
> This will make some difference in the protocols that you use.
> I have an old Tissue Tek dipper type and we use one hour for every station
> and start in 70% alcohol.
> I'd be glad to share this protocol with you if this is the kind of
> processor that you have - otherwise you will need to use less time
> especially if you use vacuum and/or heat in processing.
> I would not recommend vac and heat with the mouse tissues - they are prone
> to be a bit dry after processing anyway and these will make them worse.
> Andi
>
>
>
> At 12:58 PM 12/14/99 -0500, you wrote:
> >Hi there,
> >I have been asked by a collegue to find out if anyone out there would be
> >willing to share their protocols for the overnight machine processing of
> >mouse organs. He has just acquired a new tissue processor for test
> >development and needs a good starting point . Thanks very much for your
> >assistance!
> >b
> >
> >
> >
> .....................................................................
> : Andrea Grantham, HT           Dept. of Cell Biology & Anatomy     :
> : Sr. Research Specialist       University of Arizona               :
> : (office:  AHSC 4212)          P.O. Box 245044                     :
> : (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
> : (FAX:  520-626-2097)          (email:  histo@u.arizona.edu)       :
> :...................................................................:
>           http://www.cba.arizona.edu/histology-lab.html