RE: methyl methac.embedding

<< Previous Message | Next Message >>
From:rmoretz@rdg.boehringer-ingelheim.com (by way of histonet)
To:histonet@histosearch.com
Reply-To:
Content-Type:text/plain; charset="us-ascii"

There are disposable molds for methacrylate embedding sold by all of the EM
supply firms (SPI, Polysciences, EM Sciences, Ladd, Energy Beam Sciences,
Fullam).  If the samples are very small, the best alternative is to use
gelatin capsules.  One of the major things affecting polymerization is
exposure to oxygen, so it is important to seal the embedding mold to
minimize such exposure.  There is a host of literature available starting
with articles in Stain Technology (back in the late 60's and early 70's).  A
quick search on BIOSIS or MEDLINE (can be accessed through PUBMED on the
Internet) should result in a host of references.  Any of the suppliers of
the monomers should also have method sheets and a list of accessories.  Hope
this helps.

Roger Moretz, Ph.D.
Dept of Toxicology

> -----Original Message-----
> From:	P. Emry [SMTP:emry@u.washington.edu]
> Sent:	Tuesday, November 30, 1999 7:02 PM
> To:	HistoNet@pathology.swmed.edu
> Subject:	methyl methac.embedding
>
> Hi,
>
> A post doc in our lab is just starting to do methyl
> methacrylate,(spelling?) and is using glass vials which she breaks when
> they are done.  There has to be a better way, right?  Please let me know
> what is used for embedding and a vendor for it.
>
> Thanks,
>
> Trisha
> UW Seattle
>




<< Previous Message | Next Message >>