RE: fluorescent peroxidase substrate

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From:"Nocito, Joseph" <joseph_nocito@srhc.iwhs.org> (by way of histonet)
To:histonet@histosearch.com
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Corazon,
I think Vector has Texas Red which is similar to New Fuchsin.  I believe it
is insoluable in alcohol in xylene, is uded with alk-phos detection systems
and can be viewed under light and IF microscopes.

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas










> -----Original Message-----
> From:	Corazon Bucana [SMTP:bucana@audumla.mdacc.tmc.edu]
> Sent:	Thursday, December 02, 1999 11:11 AM
> To:	histonet@pathology.swmed.edu
> Subject:	fluorescent peroxidase substrate
>
> Just out of curiousity, is there a peroxidase substrate  for
> immunohistochemistry that forms an insoluble precipitate and at the same
> time is also fluorescent?  I am looking for something analogous to Fast
> Red
> that is used for alkaline phosphatase.  The fluorescence provides more
> sensitivity.
>
> Thank you,
>
> Corazon D. Bucana
> *******************************************************
> Corazon D. Bucana, Ph.D.
> Department of Cancer Biology
> U.T. M.D. Anderson Cancer Center
> 1515 Holcombe Blvd.  Box 173
> Houston, Texas 77030
> Phone: (713) 792-8106
> FAX: (713) 792-8747
> Email:bucana@audumla.mdacc.tmc.edu
> FAX: (713) 792-8747
>




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