Linda,
I use vegf and fgfb both from santa cruz on paraffin processed tissues. i
fix with zinc formal but i amagine 10% NBF would work, but may require more
stringent antigen retriveal.  all i do is a short proteinase k digestion (5
min.) before staining.  i use a 1:200 dilution and a strept-avidin biotin
hrp detection system (DAKO)
Patsy

-----Original Message-----
From: Sebree Linda A. [mailto:la.sebree@hosp.wisc.edu]
Sent: Thursday, December 02, 1999 10:18 AM
To: 'Histonet'
Subject: VEGF and bFGF


Hi Histonetters,

I need some help.  I'm trying to optimize Vascular Enothelial Growth Factor
(VEGF) and angiogenic factor bFGF, both from Oncogene.  I need these to work
on paraffin and a Ventana immunostainer.  If anyone has any experience out
there with these I'd appreciate some info.  I'm not wedded to these
particular antibodies, although the researcher may think we are!...so if
there are other vendor's antibodies out there that people have used with
sucess, I'd be interested in hearing about those too.

Thanks for the help,

Linda A. Sebree, HT
University of Wisconsin Hospital & Clinics
Immunohistochemistry/In Situ Hybridization Laboratory
D4/218-2472
600 Highland Avenue
Madison, WI  53792-2472


(608)265-6596
FAX: (608)263-1568