Joyce,
when we used the microwave oven to treat our slides, we placed the tissue on
positive charged slides.  The waterbath was filled with distilled water only
(no gelatin or other adhesive) and kept the slides in a 60 degree oven
overnight.  We still had some problems with bone marrows and some fatty
tissue such as breast.  we changed to steaming our slides.  We get good
results and it is less damaging to the tissue.  Hope this helps.

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas


> -----Original Message-----
> From:	Joyce Friedland [SMTP:joycefr@frontiernet.net]
> Sent:	Thursday, December 02, 1999 2:43 PM
> To:	histonet@pathology.swmed.edu
> Subject:	Tissue Adhesion for Immunos
>
> What methods are all the experts out there using to keep tissue from
> loosening, folding over and falling off during Immunoperoxidase
> procedures?? The biggest problem is during antigen retrieval using citrate
> buffer in the microwave.
>
> Thanks,
> Joyce
>
> ************************************
> *  Joyce Friedland                 *
> *  joycefr@frontiernet.net         *
> *  www.frontiernet.net/~joycefr    *
> ************************************
>
>