RE: Removal of DAB Chromogen
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From: | "Sanders, Julie, VHACIN" <Julie.Sanders@med.va.gov> (by way of histonet) |
To: | histonet@histosearch.com |
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Julie A. Sanders, BA, HT(ASCP)
Supervisor, Anatomic Pathology/Administrative Office
> -----Original Message-----
> From: Richard Cartun [SMTP:Rcartun@harthosp.org]
> Sent: Tuesday, December 14, 1999 5:19 PM
> To: Histonet@pathology.swmed.edu
> Subject: Removal of DAB Chromogen
>
> Help!
>
> We had a problem in the lab today where our cytokeratin cocktail
> (AE1&AE3/CAM5.2) was put on sections of prostate instead of the requested
> cytokeratin 34BetaE12 mAb. Is there any way to remove the DAB reaction
> product and re-stain the slides using the correct mAb? If we re-stain the
> slides do I have to be concerned with binding of the detection reagents to
> the first primary mAb?
>
> R. Cartun
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