RE: Removal of DAB Chromogen

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From:"Sanders, Julie, VHACIN" <> (by way of histonet)
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Julie A. Sanders, BA, HT(ASCP)
Supervisor, Anatomic Pathology/Administrative Office

> -----Original Message-----
> From:	Richard Cartun []
> Sent:	Tuesday, December 14, 1999 5:19 PM
> To:
> Subject:	Removal of DAB Chromogen
> Help!
> We had a problem in the lab today where our cytokeratin cocktail
> (AE1&AE3/CAM5.2) was put on sections of prostate instead of the requested
> cytokeratin 34BetaE12 mAb.  Is there any way to remove the DAB reaction
> product and re-stain the slides using the correct mAb?  If we re-stain the
> slides do I have to be concerned with binding of the detection reagents to
> the first primary mAb?
> R. Cartun

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