We don't use Ki67, but we have had success with HEIR with the Decloaking
Chamber by Biocare Medical.  There were other antibodies that we could not
get to stain until we tried this.  We use the Decloaking chamber and the
Reveal.  We have also used their Nuclear Decloaker with success.

Jennifer MacDonald

> ----------
> From: 	Kathy Oprea[SMTP:koprea@calc.vet.uga.edu]
> Sent: 	Friday, December 10, 1999 11:37 AM
> To: 	Histonet@pathology.swmed.edu
> Subject: 	Ki67 staining in canine tissue
>
> Does anyone have a reliable protocol for staining canine tissue with
> Ki67. What antigen retrieval works best? steam, autoclave, microwave?
> Which citrate buffer works best? Also which antibody are you using?
> We have tried both monoclonal and polyclonal, with several types of
> animals, but cannot get consistent results. Now there is a study
> about to begin for mast cell tumors in dogs and I need help setting
> up a protocol. You can respond to my email if you don't want to post
> on histonet. If there are vendors out there that have suggestions I
> welcome your input.
> Thank you,
> Kathy
>                 Thank you
>  Kathy Oprea
>  University of Georgia
>  College of Veterinary Medicine
>  Athens, GA 30602-7388
>  KOPREA@calc.vet.uga.edu
> The worker who's never said "Thank
> God it's Friday," probably never
> really worked.
>
>