Timely email - I am about to start using Ki67 staining,
is there some special procedure I should be aware of, is
this one of those antibodies that requires some trick and
results in sleepless nights?

I will using it in rat/mouse, a monoclonal Ab from Novocastro.

Thanks for any suggestions.
Judy

>
>> ----------
>> From: 	Kathy Oprea[SMTP:koprea@calc.vet.uga.edu]
>> Sent: 	Friday, December 10, 1999 11:37 AM
>> To: 	Histonet@pathology.swmed.edu
>> Subject: 	Ki67 staining in canine tissue
>>
>> Does anyone have a reliable protocol for staining canine tissue with
>> Ki67. What antigen retrieval works best? steam, autoclave, microwave?
>> Which citrate buffer works best? Also which antibody are you using?
>> We have tried both monoclonal and polyclonal, with several types of
>> animals, but cannot get consistent results. Now there is a study
>> about to begin for mast cell tumors in dogs and I need help setting
>> up a protocol. You can respond to my email if you don't want to post
>> on histonet. If there are vendors out there that have suggestions I
>> welcome your input.
>> Thank you,
>> Kathy
>>                 Thank you
>>  Kathy Oprea
>>  University of Georgia
>>  College of Veterinary Medicine
>>  Athens, GA 30602-7388
>>  KOPREA@calc.vet.uga.edu
>
>


Judy Trogadis
Vision Science Research Program
Toronto Western Hospital Research Inst.
399 Bathurst  St., Rm. Mc6-415
Toronto, Canada M5T 2S8

phone: 416-603-5088
Fax:   416-603-5126
email: judy@playfair.utoronto.ca