RE: Fixation of Smears

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From:"Greer, Patricia" <> (by way of histonet)
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We have used the following fixative for blood smears and cytospins prior to


Acetone		19 ml
Methanol		19 ml
Formalin, 40%	  1 ml

Fix air dried  smears or cytospins for 60 - 90 seconds.  Shake off excess
fixative and transfer slides to buffer for 5 minutes.

Patricia W. Greer, MT,HTL(ASCP)
Centers for Disease Control
Infectious Disease Pathology
Mail Stop G-32
Atlanta, GA 30333
phone 404-639-2811

-----Original Message-----
From: Bourassa, Patricia []
Sent: Tuesday, December 14, 1999 7:46 AM
To: Histonet @Pathology.Swmed.Edu (E-mail)
Subject: Fixation of Smears

Hi, all!

Not sure if this can be done, but here goes.  We want to make a blood smear
onto a slide and then fix it ASAP.  Can this be done without the smeared
cells falling off the slide?  Thanks!

-Patti Bourassa
 Pfizer, Groton

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