Patti,
We have used the following fixative for blood smears and cytospins prior to
immunostaining:

		FAM

Acetone		19 ml
Methanol		19 ml
Formalin, 40%	  1 ml

Fix air dried  smears or cytospins for 60 - 90 seconds.  Shake off excess
fixative and transfer slides to buffer for 5 minutes.

***********************
Patricia W. Greer, MT,HTL(ASCP)
Centers for Disease Control
Infectious Disease Pathology
Mail Stop G-32
Atlanta, GA 30333
phone 404-639-2811

-----Original Message-----
From: Bourassa, Patricia [mailto:patricia_bourassa@groton.pfizer.com]
Sent: Tuesday, December 14, 1999 7:46 AM
To: Histonet @Pathology.Swmed.Edu (E-mail)
Subject: Fixation of Smears


Hi, all!

Not sure if this can be done, but here goes.  We want to make a blood smear
onto a slide and then fix it ASAP.  Can this be done without the smeared
cells falling off the slide?  Thanks!

-Patti Bourassa
 Pfizer, Groton