RE: Daily Digest
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| From: | Monteverde Cheryl A <Cheryl.Monteverde@nw.amedd.army.mil> (by way of histonet) |
| To: | histonet@histosearch.com |
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Michelle,
Since you are in Anchorage, contact Dr. Probst at the State Medical
Examiner's office, I'm sure he would be able to help you obtain the tissues
you need.
-----Original Message-----
From: HistoNet Server [mailto:histonet@pathology.swmed.edu]
Sent: Tuesday, December 07, 1999 9:15 PM
To: HistoNet Server
Subject: Daily Digest
----------------------------------------------------------------------
Date: 7 Dec 1999 01:32:09 -0600
From: Kappeler Andreas <kappeler@patho.unibe.ch>
Subject: Aqueous mounting medium
Dear Histonetters
Has anybody a good suggestion (brand name, catalogue number) for an aqueous
mounting medium that i) dries completely and reasonably fast (-> slides to
be
archived); ii) doesn't start with lots of tiny air bubbles that have no
other
purpose than to grow larger with time; iii) is easily applied, i.e. no
first
layer, let dry oN, second layer etc. (our pathologists don't like to wait
...); iv) doesn't cost a fortune? We've been using Aquatex from Merck and
Aquamount Gurr from BDH with our immunohistochemistry slides (alkaline
phosphatase - new fuchsin -> better contrast with aqueous mounting medium
than
after clearing and resin-based mounting medium) for quite a while but never
were really happy with these products, in particular because of their
inherent
tendency to form bubbles. Any suggestions to make our lab life bubble-free
are
welcome! If you have experience with aqueous mounting media on automated
coverslippers, don't hesitate to let me know. Thanks a lot.
Andi Kappeler
Institute of Pathology, University of Bern
Murtenstrasse 31
CH-3010 Bern, Switzerland
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<DIV><FONT color=#000000 size=2>Dear Histonetters</FONT></DIV>
<DIV><FONT color=#000000 size=2>Has anybody a good suggestion (brand name,
catalogue number) for an aqueous mounting medium that i) dries
completely
and reasonably fast (-> slides to be archived); ii) doesn't start
with
lots of tiny air bubbles that have no other purpose than to grow larger with
time; iii) is easily applied, i.e. no first layer, let dry oN, second
layer etc. (our pathologists don't like to wait ...); iv) doesn't cost
a
fortune? We've been using Aquatex from Merck and Aquamount Gurr from BDH
with
our immunohistochemistry slides (alkaline phosphatase - new fuchsin ->
better
contrast with aqueous mounting medium than after clearing and resin-based
mounting medium) for quite a while but never were really happy with these
products, in particular because of their inherent tendency to form bubbles.
Any
suggestions to make our lab life bubble-free are welcome! If you have
experience
with aqueous mounting media on automated coverslippers, don't hesitate to
let
me
know. Thanks a lot.</FONT></DIV>
<DIV> </DIV>
<DIV><FONT color=#000000 size=2>Andi Kappeler<BR>Institute of Pathology,
University of Bern<BR>Murtenstrasse 31<BR>CH-3010 Bern,
Switzerland<BR></FONT></DIV></BODY></HTML>
- --Boundary_(ID_lr5RuNXf+pxkzHgrdD0c+g)--
----------------------------------------------------------------------
Date: 7 Dec 1999 03:16:51 -0600
From: Paul Klosen <klosen@neurochem.u-strasbg.fr>
Subject: Re: Aqueous mounting medium
Give Biomeda's Crystalmount or Shandon's Aquaperm a try. They work pretty
well. Just apply over the sections and let it dry without coverslip. After
it has dried, you can coverslip the sections with an organic solvent based
resin for permanent storage. In my hands, these are the only aqueous
mounting media that come close to organic resins in terms of refractive
index and thus section transparency. I must however say that they are too
pricey in my opinion.
As to the use of automated coverslippers with these media, I have no
experience.
Paul
-=-
(o -) O
===============================oOo==(_)==OOo================================
==
Paul Klosen, PhD
CNRS UMR 7518 Neurobiologie des Fonctions Rythmiques et Saisonnieres
Universite Louis Pasteur 12, rue de l'Universite
F-67000 Strasbourg, FRANCE
Tel. 03.88.35.85.04 Fax. 03.88.24.04.61
========================klosen@neurochem.u-strasbg.fr=======================
==
----------------------------------------------------------------------
Date: 7 Dec 1999 06:01:21 -0600
From: "kunihiro uryu" <hirouryu@hotmail.com>
Subject: Position at U of Penn
We have a Research Specialist Position available at CENTER FOR
NEURODEGENERATIVE DISEASE RESEARCH of University of Pennsylvania. This
Center is the core research center of Alzheimer Center of University of
Pennsylvania. We are looking for a motivated and enthusiastic person who is
willing to join our scientific challenge in understnding the cause and the
cure for dimentia with brain dysfunction, such as Alzheimer Disease.
Position Summary is as follow,
Assistant in research of neuronal cytoskelton in health and disease
using established and novel histological methods. Other duties include
microscopy, maintaining databases, demonstrating techniques to students and
postdoctral fellows. Maintaining equipment, ordering supplies, making stock
solutions. Writing method sections for manuscripts, preparing microscopy
images as slide and prints for publications and seminars.
To find our more about us, you can visit our web site,
http://www.med.upenn.edu/cndr/.
Feel free to send me your CV.
Kunihiro Uryu, Ph.D.
Senior Research Investigator
Center for Neurodegenerative Disease Research
Department of Pathology and Laboratory Medicine
University of Pennsylvania School of Medicine
3rd Floor Maloney Building
3600 Spruce St.
Philadelphia, PA 19104
______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com
----------------------------------------------------------------------
Date: 7 Dec 1999 07:15:42 -0600
From: Becky LoVerso <loverso-toth.1@osu.edu>
Subject: staining old tissue
Hi. I've got some mouse spinal cord tissue that has been sitting on slides
in a freezer for two years. I need to stain it for oligodendricytes with a
cc-1 antibody. The procedure that was prepared workes beautifully on
tissue that has been UNCUT in a freezer for a long time, and was cut only
recently to practice for this stain. However, when the precut old tissue
(the important stuff) is used with the same procedure, the background
staining is terribly high. Oh yeah, it's developed with DAB. The
oligodendricytes are too light to be seen clearly against the background
stain, making them uncountable. Our head histotech thinks its due to
freezer burn, or something similar to it. Does anyone have any ideas as to
how I can salvage the rest of this tissue?
Thanks,
Rebecca LoVerso
Dept. of Neuroscience
The Ohio State University
----------------------------------------------------------------------
Date: 7 Dec 1999 08:15:59 -0600
From: "kunihiro uryu" <hirouryu@hotmail.com>
Subject: Position @ U of Penn
We have a Research Specialist Position available at CENTER FOR
NEURODEGENERATIVE DISEASE RESEARCH of University of Pennsylvania. This
Center is the core research center of Alzheimer Center of University of
Pennsylvania. We are looking for a motivated and enthusiastic person who is
willing to join our scientific challenge in understnding the cause and the
cure for dimentia with brain dysfunction, such as Alzheimer Disease.
Position Summary is as follow,
Assistant in research of neuronal cytoskelton in health and isease
using established and novel histological methods. Other duties include
microscopy, maintaining databases, demonstrating techniques to students and
postdoctral fellows. Maintaining equipment, ordering supplies, making stock
solutions. Writing method sections for manuscripts, preparing microscopy
images as slide and prints for publications and seminars.
To find our more about us, you can visit our web site,
http://www.med.upenn.edu/cndr/.
Feel free to send me your CV.
Kunihiro Uryu, Ph.D.
Senior Research Investigator
Center for Neurodegenerative Disease Research
Department of Pathology and Laboratory Medicine
University of Pennsylvania School of Medicine
3rd Floor Maloney Building
3600 Spruce St.
Philadelphia, PA 19104
______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com
----------------------------------------------------------------------
Date: 7 Dec 1999 08:46:07 -0600
From: Inka Tertinegg <inka@playfair.utoronto.ca>
Subject: ASCP test
A microcurie is a measure of radioactivity, a millionth of a Curie
and
37,000 disintegrations per second.
Inka Tertinegg
University of Toronto, Department of Ophthalmology
399 Bathurst St., rm Mc6-411
Toronto, Ontario
M5T 2S8
(416)603-5800 ext 2850
----------------------------------------------------------------------
Date: 7 Dec 1999 08:58:46 -0600
From: "Carson, Karla" <KCarson@chw.edu>
Subject: Microcure
Thanks to all who responded to the microcurie question. Jason says he can't
remember all the answers given but is sure that they did not simply indicate
anything to do with a curie. Anyway, it was an interesting question and
another indication that histology is expanding.
Karla
----------------------------------------------------------------------
Date: 7 Dec 1999 10:00:58 -0600
From: portera@att.net
Subject: dura edge inquiries
Blades can also be ordered through:
Source Medical Products Inc.
1274 Telegraph Road
Lake Forest, IL 60045
PH: 847-735-9965
Fax: 847-735-0366
Federal ID: 36-4250405
Hope this helps someone out.
----------------------------------------------------------------------
Date: 7 Dec 1999 11:30:57 -0600
From: Sharon Bledsoe <sbledsoe@iupui.edu>
Subject: A0
We have an old AO microtome.... who purchased them???
----------------------------------------------------------------------
Date: 7 Dec 1999 11:47:12 -0600
From: johnsom@shmc.org (Mickie L. Johnson)
Subject: Trying to Reach Lauren
Lauren, will you contact me again. Your return email address does not work.
Please include your email address in your reply.
Thanks
Mickie
Michael L Johnson, BS, HTL(ASCP)
Histology Supervisor
Department of Pathology
Sacred Heart Medical Center
W. 101 8th Avenue
Spokane, WA 99220
johnsom@shmc.org
----------------------------------------------------------------------
Date: 7 Dec 1999 12:46:03 -0600
From: Sharon Bledsoe <sbledsoe@iupui.edu>
Subject: Re: AO-Thanks for the quick responses
Thanks to all for the quick responses.
Sharon
----------------------------------------------------------------------
Date: 7 Dec 1999 13:01:25 -0600
From: "Michael J. Lyon, Ph.D." <lyonm@VAX.CS.HSCSYR.EDU>
Subject: Tyrosine hydroxylase and dopamine Beta hydroxylase
I have been searching Linscott's for sources of Tyrosine hydroxylase and
dopamine Beta hydroxylase antibodies and there are just to many sources and
variations so I thought I would post this inquiry to you more experienced
netters. I would like to get some of your experiences with either Tyrosine
hydroxylase and dopamine Beta hydroxylase antibodies: sources etc. It would
be good if these cross react in humans and rats but human reactivity is the
most important. Also, the tissues will most likely be formalin fixed some
whole mount vascular elements as well as frozen sections.
Thanks
Mike
Michael J. Lyon, Ph.D.
Associate Professor Otolaryngology
315-464-7253 Voice
315-464-5572 FAX
----------------------------------------------------------------------
Date: 7 Dec 1999 13:15:37 -0600
From: rschoonh@sph.unc.edu
Subject: Re: A0
Sharon,
The quick answer to that question is Leica 800-248-0123. The long
answer may explain why I feel that it is doubtfull if they will have AO
(American Optical) parts available. They have gone through several name
changes (AO Reichert, Reichert-Jung, Cambridge Inst., and Leica) and one
major location change, from Buffalo, NY to Chicago, IL. I left the
company shortly after Cambridge Inst.'s took over and at that time there
were still some parts available in Buffalo for the AO Spencer microtomes
but not a lot. That has been some years ago so there is no telling
what, if any, of the older parts are still available.
best regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone
office 919-966-6343
Lab 919-966-6140
Fax 919-966-6123
Don't go around saying the world owes you a living; the world owes you
nothing; it was here first.
Mark Twain [Samuel Langhornne Clemens] (1835-1910)
- -- Begin original message --
> From: Sharon Bledsoe <sbledsoe@iupui.edu>
> Date: Tue, 07 Dec 1999 12:11:53 -0500
> Subject: A0
> To: histonet@pathology.swmed.edu
>
> We have an old AO microtome.... who purchased them???
>
>
- -- End original message --
----------------------------------------------------------------------
Date: 7 Dec 1999 13:16:06 -0600
From: Mary Lou Norman <mlm11@cornell.edu>
Subject: Re: AO-Thanks for the quick responses
IWhat responses? I still don't know who bought AO.
At 13:45 1999-12-07 -0500, you wrote:
>Thanks to all for the quick responses.
>
>Sharon
>
>
----------------------------------------------------------------------
Date: 7 Dec 1999 13:42:51 -0600
From: "Carol Bobrowitz" <Carol_Bobrowitz.PATHOLOGY@qmail.path.mcw.edu>
Subject: Minneapolis Vendor
Subject: Time: 12:34 PM
OFFICE MEMO Minneapolis Vendor Date: 12/7/99
A histology technician within the MCW system is looking for a vendor, in
Minneapolis, who sells used equipment.
Please call Kathy at (414) 456-4073.
Thank you,
Carol Ann Bobrowitz
Medical College of Wisconsin
Milwaukee, Wisconsin
----------------------------------------------------------------------
Date: 7 Dec 1999 13:43:17 -0600
From: "Instrumedics, Inc." <cfss@idt.net>
Subject: CureMount mounting medium
Many of you have asked about a UV lamp to use for the new curable mounting
medium, CureMount.
Many of you have a UV lamp, but for those who don't, we will be offering an
inexpensive lamp that will have a tray that holds 15 slides and fit neatly
under the UV light.
For the details please contact Instrumedics
800-237-2772 or by e-mail
If any of you have not received the sample of CureMount you requested please
let us know and we will be sure to get one to you.
We will exhibiting at the annual meeting of the American Society for Cell
Biology at the Convention Center in DC, Dec.12-15.. We invite you to visit
our
booth # 236 for a demonstration of the CryoJane Tape-Transfer system,
Cryo-Vac-away, CureMount and several other technologies.
If you need a guest pass we can get one for you.
Bernice
schiller@instrumedics.com
----------------------------------------------------------------------
Date: 7 Dec 1999 14:15:07 -0600
From: FreidaC@aol.com
Subject: Re: Reigistry exam
Jo Ann:
The registry exam is different for each person. Since the exam is computer
administered, it can easily determine each person's ability. The first
question given will be of moderate difficulty. If you get it right, the
next
question will be more difficult, but if you get it wrong the next question
will be easier. Thus each question is determined by your response on the
previous question. There is a preset pass/fail point, and you must end up
above this point to pass the exam. That is a very simplistic answer to your
question, but hopefully will explain why each person gets a different exam,
unless two people respond to all questions in exactly the same manner.
Freida Carson
----------------------------------------------------------------------
Date: 7 Dec 1999 15:45:54 -0600
From: "MacDonald, Jennifer" <jmacdonald@sach.org>
Subject: RE: A0
Are there specific parts that you are looking for? I have a few odds and
ends in a drawer here. We used to have two AOs but sold them.
Jennifer
> ----------
> From: rschoonh@sph.unc.edu[SMTP:rschoonh@sph.unc.edu]
> Sent: Tuesday, December 07, 1999 12:19 PM
> To: sbledsoe@iupui.edu; histonet@pathology.swmed.edu
> Subject: Re: A0
>
> Sharon,
>
> The quick answer to that question is Leica 800-248-0123. The long
> answer may explain why I feel that it is doubtfull if they will have AO
> (American Optical) parts available. They have gone through several name
> changes (AO Reichert, Reichert-Jung, Cambridge Inst., and Leica) and one
> major location change, from Buffalo, NY to Chicago, IL. I left the
> company shortly after Cambridge Inst.'s took over and at that time there
> were still some parts available in Buffalo for the AO Spencer microtomes
> but not a lot. That has been some years ago so there is no telling
> what, if any, of the older parts are still available.
>
> best regards,
> Bob
> Robert Schoonhoven
> Laboratory of Molecular Carcinogenesis and Mutagenesis
> Dept. of Environmental Sciences and Engineering
> University of North Carolina
> CB#7400
> Chapel Hill, NC 27599
> Phone
> office 919-966-6343
> Lab 919-966-6140
> Fax 919-966-6123
>
> Don't go around saying the world owes you a living; the world owes you
> nothing; it was here first.
> Mark Twain [Samuel Langhornne Clemens] (1835-1910)
>
> -- Begin original message --
>
> > From: Sharon Bledsoe <sbledsoe@iupui.edu>
> > Date: Tue, 07 Dec 1999 12:11:53 -0500
> > Subject: A0
> > To: histonet@pathology.swmed.edu
> >
> > We have an old AO microtome.... who purchased them???
> >
> >
>
> -- End original message --
>
>
----------------------------------------------------------------------
Date: 7 Dec 1999 15:46:30 -0600
From: Geoff McAuliffe <mcauliff@UMDNJ.EDU>
Subject: Re: Tyrosine hydroxylase and dopamine Beta hydroxylase
"Michael J. Lyon, Ph.D." wrote:
> I have been searching Linscott's for sources of Tyrosine hydroxylase and
> dopamine Beta hydroxylase antibodies and there are just to many sources
and
> variations so I thought I would post this inquiry to you more experienced
> netters. I would like to get some of your experiences with either
Tyrosine
> hydroxylase and dopamine Beta hydroxylase antibodies: sources etc. It
would
> be good if these cross react in humans and rats but human reactivity is
the
> most important. Also, the tissues will most likely be formalin fixed some
> whole mount vascular elements as well as frozen sections.
>
> Thanks
>
> Mike
>
> Michael J. Lyon, Ph.D.
> Associate Professor Otolaryngology
> 315-464-7253 Voice
> 315-464-5572 FAX
I have had great success with antibodies to TH and DBH from Eugene Tech.
on
formalin-fixed frozen or cryostat sections of rat or mouse tissues. I use
the
Vector or Vector Elite kits for detection.
My impression is that there are several (or more) companies supplying
good
antibodies to these enzymes.
Geoff
- --
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
**********************************************
----------------------------------------------------------------------
Date: 7 Dec 1999 16:11:42 -0600
From: "Ford M. Royer" <froyer@bitstream.net>
Subject: Re: Minneapolis Vendor
Please contact me at the numbers listed below.
Local phone is 612-929-1996
I specialize in Histology/Cytology Refurbished equipment and am located in
Minneapolis, MN
- --
Ford M. Royer, MT(ASCP)
Analytical Instruments, Ltd
(Refurbished Histology, Cytology, & General Lab Equipment)
9921 13th Ave. N.
Minneapolis, MN 55441-5004
800-565-1895 phone
612-929-1895 fax
web site: http://www.aibltd.com
----------------------------------------------------------------------
Date: 7 Dec 1999 16:12:13 -0600
From: "Mary L. Verlinde" <verlinde@ahdlms.cvm.msu.edu>
Subject: JB4 Microtome
I am asking a question for one of our pathologists. Does anyone know of a
JB-4 microtome? This is the only reference he has to this machine. Who makes
this particular model? Thanks in advance.
Mary Verlinde
Animal Health Diagnostic Lab
MSU
----------------------------------------------------------------------
Date: 7 Dec 1999 16:45:18 -0600
From: "Donna Sitrin" <dsitrin@unipathllc.com>
Subject: grossing techs
In regard to employees who gross in small tissues, does anyone have any idea
what pay scales are being used out there?
I have employees who gross in small bxs, such as GI, ECC, Skins, breast
cores, etc. They have the required education and experience per Clia
to do this. We have been dealing with employee councils to see how our
scales measure up, but there is nothing that even matches their job
description on which to base a comparison. They are not histotechs.
Any ideas??
Thanks in advance,
Donna
dsitrin@unipathllc.com
----------------------------------------------------------------------
Date: 7 Dec 1999 17:30:51 -0600
From: "Skelton, Michelle" <mskelton@anthc.org>
Subject: HT Practical
When doing the practical for the HT exam, the rules say you can use
automated equipment - Does this include the automated coverslipper? I am
assuming that it does. Also, does anybody have any spare spinal cord or
stomach laying around that they are not using? Thanks in advance.
Michelle Skelton
Alaska Native Medical Center
Anchorage, AK
----------------------------------------------------------------------
Date: 7 Dec 1999 17:44:37 -0600
From: larisonk@uoneuro.uoregon.edu
Subject: Innovex for blocking
Histonetters,
Someone at the NSH meeting mentioned Innovex blocking solution. Does anyone
know
where I can obtain this reagent?
Karen in Oregon
----------------------------------------------------------------------
Date: 7 Dec 1999 17:59:21 -0600
From: kathy_hicks_at_opr-2@smtp.mcis.uchicago.edu
Subject: meeting
May 18th and 19th 2000
Illinois State Meeting
Schaumburg, Illinois
Radisson Hotel
How would you like to win a free registration to the NSH meeting in
Milwaukee, Wisconsin??? Well, the year 2000 meeting is going to offer
that possibility to each and every one of you!! Upon registration for
our two day meeting your name will be submitted for the drawing, which
will take place on Friday May 19th at afternoon break in the
exhibitors hall.
The meeting is just a two day event, but guarantees to stimulate and
educate you and hopefully give you a little something to take back to
your lab to improve things for you and your peers. Our very own Judy
Bordyn, sponsored by SAKURA, is making a beautiful hand-made quilt to
be raffled off during the meeting. There are alot of special things
planned for this one as we celebrate the year 2000 and once again
commit to histological excellence in all of our laboratories.
Announcements for registration to this (not to be missed) event will
be coming your way soon!!
Please feel free to contact our President, Cathy Locallo at
7730702-8492, or the secretary, Kathy Hicks at 773-702-9052.
clocallo@mcis.bsd.uchicago.edu
or
khicks@mcis.bsd.uchcago.edu
----------------------------------------------------------------------
Date: 7 Dec 1999 19:01:07 -0600
From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
Subject: JB-4 microtome
The JB-4 is sold by Energy Beam Sciences, Stephen Slap, at 800 992-9037.
Gayle Callis
----------------------------------------------------------------------
Date: 7 Dec 1999 19:12:52 -0600
From: Patsy.Ruegg@UCHSC.edu
Subject: RE: JB4 Microtome
The sorvall jb4 microtome was orginally made by Dupont many years ago, used
for plastic sectioning mainly but can be adapted to cut paraffin. i have 4
of them and love them, i keep them all around for spare parts. i think
Energy Beam Sciences sells them now.
Patsy
- -----Original Message-----
From: Mary L. Verlinde [mailto:verlinde@ahdlms.cvm.msu.edu]
Sent: Tuesday, December 07, 1999 5:10 PM
To: 'HISTONET@PATHOLOGY.SWMED.EDU'
Subject: JB4 Microtome
I am asking a question for one of our pathologists. Does anyone know of a
JB-4 microtome? This is the only reference he has to this machine. Who makes
this particular model? Thanks in advance.
Mary Verlinde
Animal Health Diagnostic Lab
MSU
----------------------------------------------------------------------
Date: 7 Dec 1999 19:13:22 -0600
From: "R.Wadley" <s9803537@pop3.unsw.edu.au>
Subject: Re: staining old tissue
Dear Rebecca,
One of the great problems with precutting & storing tissue is that
you
expose the tissue to oxidation (sp) & reduction reactions with the
surrounding environment. This is also true of blocks. However, by the
time you have trimmed the surface of a block you are into the unaffected
tissue. While there are many people who can report that they have stored
unstained slides forever without detrimental effect, it depends on the
staining. H&E no problem, many histochemical & some immuno staining - no
problem. BUT, for the really important stuff - big problems. In some
cases the chemical reactions that have taken place can be reversed, ie
antgen retrieval (if your lucky). I was taught to always store blocks, &
only store unstained slides for short periods (weeks to months depending on
what they were for). Refrigeration slows chemical reactions it doesn't
prevent them. If you must store unstained slides & must get good results
from them I can only suggest placing them immediately in a container filled
with nitrogen gas, temperature of storage - probably cold.
Long term storage of tissue in fixative creates similar problems.
Regards
Rob W.
At 07:48 12/07/1999 -0500, you wrote:
>Hi. I've got some mouse spinal cord tissue that has been sitting on slides
>in a freezer for two years. I need to stain it for oligodendricytes with a
>cc-1 antibody. The procedure that was prepared workes beautifully on
>tissue that has been UNCUT in a freezer for a long time, and was cut only
>recently to practice for this stain. However, when the precut old tissue
>(the important stuff) is used with the same procedure, the background
>staining is terribly high. Oh yeah, it's developed with DAB. The
>oligodendricytes are too light to be seen clearly against the background
>stain, making them uncountable. Our head histotech thinks its due to
>freezer burn, or something similar to it. Does anyone have any ideas as to
>how I can salvage the rest of this tissue?
>Thanks,
>Rebecca LoVerso
>Dept. of Neuroscience
>The Ohio State University
R. Wadley, B.App.Sc. M.L.S, Grad.Dip.Sc.MM
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW, New South Wales, Australia, 2052
Ph (BH) +61 (2) 9385 3517
Ph (AH) +61 (2) 9555 1239
Fax +61 (2) 9385 1591
E-mail r.wadley@unsw.edu.au
www http://www.micro.unsw.edu.au/caf.html
----------------------------------------------------------------------
Date: 7 Dec 1999 19:13:55 -0600
From: Paul Popper <ppopper@mcw.edu>
Subject: cgrp antibody
Does anybody know of a CGRP antibody directed to the N-terminus?
Thanks
Paul
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- --------------034C1BEB0E745A6357076C54--
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Date: 7 Dec 1999 19:47:25 -0600
From: "Mark & Carrie Byrne" <eire@teleport.com>
Subject: RE: HT Practical
michelle,
using an automated coverslipper is ok as long as it uses GLASS coverslips.
the instructions specifically state that coverglasses must be used....the
tape-type are not acceptable. i know a couple of students who had their
slides rejected outright because of just this misunderstanding.
good luck,
carrie kyle-byrne
----------------------------------------------------------------------
Date: 7 Dec 1999 19:48:04 -0600
From: Amos Brooks <atbrooks@snet.net>
Subject: Re: HT Practical
Hi,
In regards to the idea of using an automatic coverslipper for your HT
practical exam. My question is WHY would you want to trust an automatic
coverslipper with your slides. Try for perfection not quantity.
Amos Brooks
"Skelton, Michelle" wrote:
> When doing the practical for the HT exam, the rules say you can use
> automated equipment - Does this include the automated coverslipper? I am
> assuming that it does. Also, does anybody have any spare spinal cord or
> stomach laying around that they are not using? Thanks in advance.
>
> Michelle Skelton
> Alaska Native Medical Center
> Anchorage, AK
----------------------------------------------------------------------
Date: 7 Dec 1999 19:48:50 -0600
From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
Subject: AO-820, repairs?
My last inquiry about an AO 820 repair ended up with IMEB used equipment
dealer, who had these instruments (a bunch, if I recall) sitting in a back
room. I don't think parts are made anymore, a repair can be done,
not cheap!and a considerable down payment on a new microtome!) and I think
parts may be scavenged off the older machines in some cases. I don't think
Leica even bothers with them anymore. This was about
to happen with my one good 820 and an old 820 no longer usable on my shelf,
I opted for a new microtome. Was difficult to give up the old workhorse,
but I had to put it out to pasture for a new thoroughbred!
Gayle Callis
----------------------------------------------------------------------
Date: 7 Dec 1999 22:53:38 -0600
From: "R.Wadley" <s9803537@pop3.unsw.edu.au>
Subject: Re: AO-820 & Spencer 880s
Dear Gayle,
I used AO's at the Uni of Tasmania & occassionally still find them
hard at
work in small/old labs. The Anatomy Dept at the Uni of Sydney still wheels
them out (& Spencers too) for students.
But in a lab where I worked about 2 years ago they had a beautiful
black
Spencer-880. The Spencer cut rings around the (newer) AO-820 that sat
beside it on the bench. As I re-equipped the lab both of the microtomes
were replaced with (2nd hand) Leica 1512s. The Spencer now has a home in
my shed as part of a collection of early microtomes, microscopes & other
histological sundries (they take up less room than vintage Buicks).
Of course the old microtomes had plenty of crosses against them.
They are
heavy to shift & often stiff (in comparison to modern machines) to operate.
Modern safety devices were missing too (& motors!). I don't always like
the modern microtomes with their light weight (ir)replacable innards, but
my arm does not ache as much when I have to do some sectioning.
Regards
Rob W.
At 16:55 12/07/1999 -0700, you wrote:
>My last inquiry about an AO 820 repair ended up with IMEB used equipment
>dealer, who had these instruments (a bunch, if I recall) sitting in a back
>room. I don't think parts are made anymore, a repair can be done,
>not cheap!and a considerable down payment on a new microtome!) and I think
>parts may be scavenged off the older machines in some cases. I don't think
>Leica even bothers with them anymore. This was about
>to happen with my one good 820 and an old 820 no longer usable on my shelf,
>I opted for a new microtome. Was difficult to give up the old workhorse,
>but I had to put it out to pasture for a new thoroughbred!
>Gayle Callis
R. Wadley, B.App.Sc. M.L.S, Grad.Dip.Sc.MM
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW, New South Wales, Australia, 2052
Ph (BH) +61 (2) 9385 3517
Ph (AH) +61 (2) 9555 1239
Fax +61 (2) 9385 1591
E-mail r.wadley@unsw.edu.au
www http://www.micro.unsw.edu.au/caf.html
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