RE: Aqueous mounting medium

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From:"Greer, Patricia" <> (by way of histonet)
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We use DAKO's FARAMOUNT for our immunos (alkaline phosphatase and fast red)
which will dry within minutes for viewing.  However we allow the slides to
dry for a couple of days beforing filing.  We had used Aquamount in the past
but began to have a problems  with the hematoxylin fading after a few days.
We keep the Faramount (which comes in a 15ml dropper bottle) upside down all
the time which helps to avoid bubbles (it fits nicely in a styrofoam 15ml
centrifuge tube holder).   We don't have any experience using it on an
automated coverslipper unfortunately.  We have the Leica coverslipper which
theoretically could use an aqueous mounting medium, but then it would have
to be dedicated to that medium and couldn't be switched back to the xylene
based.  Of course you could always get a second coverslipper.
We pay 13.50 per bottle for the Faramount.  A final comment is that this
mountant is suitable for excellent photomicography.

Pat Greer

Patricia W. Greer, MT,HTL(ASCP)
Centers for Disease Control
Infectious Disease Pathology
Mail Stop G-32
Atlanta, GA 30333
phone: 404-639-2811

-----Original Message-----
From: Kappeler Andreas []
Sent: Tuesday, December 07, 1999 2:14 AM
To: Histonet
Subject: Aqueous mounting medium

Dear Histonetters
Has anybody a good suggestion (brand name, catalogue number) for an aqueous
mounting medium that  i) dries completely and reasonably fast (-> slides to
be archived);  ii) doesn't start with lots of tiny air bubbles that have no
other purpose than to grow larger with time;  iii) is easily applied, i.e.
no first layer, let dry oN, second layer etc. (our pathologists don't like
to wait ...);  iv) doesn't cost a fortune? We've been using Aquatex from
Merck and Aquamount Gurr from BDH with our immunohistochemistry slides
(alkaline phosphatase - new fuchsin -> better contrast with aqueous mounting
medium than after clearing and resin-based mounting medium) for quite a
while but never were really happy with these products, in particular because
of their inherent tendency to form bubbles. Any suggestions to make our lab
life bubble-free are welcome! If you have experience with aqueous mounting
media on automated coverslippers, don't hesitate to let me know. Thanks a

Andi Kappeler
Institute of Pathology, University of Bern
Murtenstrasse 31
CH-3010 Bern, Switzerland

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