Giemsa stain for malaria

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From:"Hooker, Anna" <> (by way of histonet)
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Does anybody have a good recipe for Giemsa stain for malaria?
We are experiencing a lot of precipitate after multiple filtrations...
At the moment we use the alcohol-glycerol method, pH 7.5 buffer, and powder
stain from Sigma.
Is there a trick about dissolving the dye that we may be missing?
Thanks a lot,
Ana Hooker
ARUP Laboratories
Univ. of Utah
Salt Lake City <>

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