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From:denise M m Long-Woodward <> (by way of histonet)
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Hi knowledgable Histonet surfers!   One of my PostDocs wants me to ask
what other researchers are doing to label endothelial cells in mouse
tissues (frozen and/or paraffin). Please be specific about company,
clone, dilution, digestion/HIER techniques used.......etc.  We have been
using Pharmigen  Rat anti-mouse CD31 (clone MEC 13.3) and
mouse anti-human CD31 (WM59) with success but with additional lymphocytic
populations also labelling.   We have tried Ulex europeus (Vector,
Biotinylated) but it doesn't seem to label mouse vessels, only human.
Any suggestions would be appreciated.
denise woodward
boston, ma

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