Aqueous mounting medium
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From: | Kappeler Andreas <kappeler@patho.unibe.ch> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Dear Histonetters Has anybody a good suggestion (brand name,
catalogue number) for an aqueous mounting medium that i) dries completely
and reasonably fast (-> slides to be archived); ii) doesn't start with
lots of tiny air bubbles that have no other purpose than to grow larger
with time; iii) is easily applied, i.e. no first layer, let dry oN,
second layer etc. (our pathologists don't like to wait ...); iv) doesn't
cost a fortune? We've been using Aquatex from Merck and Aquamount Gurr
from BDH with our immunohistochemistry slides (alkaline phosphatase - new
fuchsin -> better contrast with aqueous mounting medium than after
clearing and resin-based mounting medium) for quite a while but never were
really happy with these products, in particular because of their inherent
tendency to form bubbles. Any suggestions to make our lab life bubble-free
are welcome! If you have experience with aqueous mounting media on
automated coverslippers, don't hesitate to let me know. Thanks a lot.
Andi KappelerInstitute of Pathology, University of BernMurtenstrasse
31CH-3010 Bern, Switzerland
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