Re: sectioning thick frozens

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From:Alan Bright <> (by way of histonet)
To:histonet <>
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I cannot understand why so many Histonetters are having problems and
avoiding thick frozen sections ?

The main points to follow to achieve thick frozen sections  are:

1) Correct tissue temperature is most important, to cold will cause
excessive cracking.

2) Brain must be sectioned between -8 to -12deg.C. & the knife temperature
should be around -10deg.C. colder than the tissue. By using this method
sections are cut up to 300 microns routinely.

Merry Christmas to All,

Alan Bright

Bright Instrument Co.Ltd.
St Margarets Way
PE18 6EB

Tel No; 01480 454528
Fax No;01480 456031
Email ;

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