Re: denatured alcohol reply

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From:Jeff Silverman <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

My reagent alcohol (Fisher says 90% Ethanol, 5% methanol, and 5%
isopropanol.  I use it for processing and staining but when I need alcohol
for compounding special stain solutions, I use 100% ethanol from pharmacy.
The reagent alcohol works fine for my eosin-phloxine counterstain though.
Happy New Year to all.
Jeff Silverman

> From: Gayle Callis <>
> To:
> Subject: denatured alcohol reply
> Date: Thursday, December 31, 1998 12:42 PM
> Alcohol labelled as "Reagent alcohol" is a denatured alcohol.  You may
> to dig around in some MSDS sheets for your "denatured alcohol" and ask
> for or call technical services at Fisher to find out what their Reagent
> alcohol contains.   If you get lucky, the container actually tells you
> is in these alcohols, often a mixture of methanol, ethyl alcohol, and
> maybe some isopropyl, or maybe someone will be able to give the info
> via H'net so you can compare and use if it is comparable to "reagent
> alcohol".
> I know people who use Reagent alcohol in processing and staining.  I
> avoid using it for dissolving dyes that are listed as 1% eosin Y in
> learned years ago this meant ethyl alcohol, when the dye did not go into
> solution.
> Happy New Year
> Gayle Callis

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