Re: autofluorescence
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From: | vandeplas@aurion.nl (Peter van de Plas) (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Ethan Hofmann wrote:
>I don't know if anybody's seen this yet, but I'm looking for a way to
>reduce or quench endogenous autofluorescence in murine GALT (gut associated
>lymphoid tissue). Are there any successful techniques for this out there?>
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Dear Ethan,
On aldehyde fixed specimens i always use 0.1% sodium borohydrid in PBS for
15 minutes. This treatment not only quenches free aldehyde groups but also
autofluorescence.
Kind regards, Peter
========================================
Peter van de Plas
AURION
Costerweg 5
6702 AA Wageningen
The Netherlands
phone: (31)-317-497676
fax: (31)-317-415955
http://www.aurion.nl
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