Re: autofluorescence

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Ethan Hofmann wrote:
>I don't know if anybody's seen this yet, but I'm looking for a way to
>reduce or quench endogenous autofluorescence in murine GALT (gut associated
>lymphoid tissue).  Are there any successful techniques for this out there?>
Dear Ethan,
On aldehyde fixed specimens i always use 0.1% sodium borohydrid in PBS for
15 minutes. This treatment not only quenches free aldehyde groups but also
Kind regards, Peter

Peter van de Plas
Costerweg 5
6702 AA Wageningen
The Netherlands
phone: (31)-317-497676
fax: (31)-317-415955

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