> Marilyn,
>                 I would agree with Sarah that this sounds as if it is
>probably
> not a problem of chatter with thick and thin areas of tissue but likely to be
> undulations in the section due to some areas lifting from the slide. This
> artifact can be especially  noticeable with thick sections and with tissue
> containing large amounts of collagen. Collagen tends to shrink  during tissue
> processing and to absorb water and expand during section mounting. This
>process
> of shrinkage and reexapansion is not necessarily shared by other tissue
>such as
> epithelia. This frequently results in folds in epithelium covering the
>connective
> tissue and in some areas the separation of the collagen from the underlying
> microscope slide. I would suggest a thiun coating of celloidin before
>staining.
> Barry.
>
> Sarah Christo wrote:
>
> > Dear Marilyn,
> >   If chatter is not your problem, I have seen this
> > happen when sections come loose from the slides
> > during staining.
> > Sarah, Texas A&M
> >
> > On Mon, 14 Dec 1998, Marilyn Woods wrote:
> >
> > > Looking for suggestions on the cause of wavy sections, so bad that we are
> > > unable to focus under high power.  This has happen out of the blue
>without
> > > changing any procedures.  I am cutting all skin, some look good,  but
>this
> > > problem seems to affect the staining ex. muddy appearing, poor
>contrast and
> > > understaining.  All temps. are with norm. except of a paraffin bath on
> > > processor is 64 C. Sections have been good 2 days a week then
>progressively
> > > get worse.
>
>   ------------------------------------------------------------------------
>
>   Barry Rittman
>   Associate Professor
>   University of Texas HSC-Dental Branch
>   Basic Sciences
>
>   Barry Rittman
>   Associate Professor
>   University of Texas HSC-Dental Branch
>   Basic Sciences
>   UT-HHSC-DB 3.112F 6516 John Freeman Avenue  Fax: (713)-500-4500
>   Houston, TX 77030                           Work: (713)-500-4134
>   Additional Information:
>   Last Name     Rittman
>   First Name    Barry
>   Version       2.1

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