Dear Katie,
treat all solutions as being light sensitive at all times. Myeloreoxidase
Rgts. are best kept in the fridge even buffers. I use a stabiliity of 3-6
months and like you add the peroxide just before use.
All haematoxylin solutions once filtered are usually free from further ppte.
exceptions would be Harris.
Regards MIke Rentsch (Downunder)
-----Original Message-----
From: Katie Bennett <bresee@pilot.msu.edu>
To: HistoNet Server <HistoNet@Pathology.swmed.edu>
Date: Saturday, 19 December 1998 3:03
Subject: Myeloperoxidase Staining


>Is there anyone out there is histoland who has used a myeloperoxidase
>stain?  I'm using the method from the Armed Forces Institute of
>Pathology handbook which calls for a peroxidase indicator reagent
>solution and an acid hematoxylin solution.  My biggest question is how
>the solutions should be stored.  Are they light sensitive?  Can they be
>re-used?  Or do they need to be used as soon as they are made up?  Does
>the Acid Hematoxylin need to be filtered before using (like other
>hematoxylin solutions I've used before)?  Also, it's been my experience
>that peroxide solutions are not stable and should be used fresh, so I
>made up the peroxidase solution in advance, minus the H2O2, which was
>added right before using.  Any advice/suggestions/experience would be
>appreciated!
>
>Thanks in advance!
>
>
>Catherine "Katie" Bresee Bennett
>218G Food Safety Toxicology Building
>Department of Pathology
>Michigan State University
>East Lansing, MI 48824
>
>ph:  (517) 432-4940
>fx:   (517) 353-9902
>
>bresee@pilot.msu.edu
>
>
>