Re: [IHC on frozen sections of brain]

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From:"Barry, Lilith" <Lilith.Barry@nrc.ca> (by way of histonet)
To:histonet <histonet@magicnet.net>
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I use Vector ABC kit routinely on perfused brains and it works very well.
The few times I have tried to use it on fresh-frozen sections, the
background was very high. Does anybody know why?
 Lilith

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From: Carol Burden
To: HistoNet@Pathology.swmed.edu
Subject: Re: [IHC on frozen sections of brain]
Date: Wednesday, December 16, 1998 6:26PM

HistoNet@Pathology.swmed.edu wrote:

Hello Everyone,
Does any of you have a tried and proven protocol for IHC on frozen brain
sections using preferably the vector ABC-HRP system or any ABC staining
system?

your prompt reply will be greatly appreciated.

Thanking you in advance.
Atoska S.Gentry,B.S, HT(ASCP)


I'd like this info as well,could you please forward your findings.
Sincerely Thanks,Carol at Emory


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