Re: Fixative

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From:rschoonh@sph.unc.edu (by way of histonet)
To:histonet <histonet@magicnet.net>
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Mary Kay,

I am assuming that you are reffering to smears?  For that we use cold
methanol (100%).

-- Begin original message --

> To All;
>   Do you think the best fixative for immunos is Acetone no matter what
> antibody one is using or is their a beter one. Does it definitely make
> a difference as to the antibody?
>    Thanks for your input.
>         Mary Kay Olson
>         CBN and Anatomy
>         Loyola University Med. Center
>         molson2@luc.edu
>
>

-- End original message --

regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123

**I'm willing to make the mistakes if someone else is willing to learn
from them**




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