Dear all:  embedding after solidified paraffin---hqave done this
routinely---everything works great, including immunos.  also, as fas as
osmium---it does enhance DAB ----but is nasty, nasty, nasty!!!be
careful--I ended up not believing it was worth the risk----just rinse
longer in gently running dh2o to clear background more.


louise



On Wed, 16 Dec 1998, HistoNet Server wrote:

>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 00:13:34 -0600
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> Subject: embedding in paraffin after RT
>
> Had a friend who did just that in a teaching situation,  without any problems
> with cutting.  I was a bit surprised that they embedded the infiltrated
> tissues after they had allowed the paraffin to harden, but it seemed to
> work.  I think one should look out for bubbles forming around the cooler
> infiltrated tissue, but if one was careful, why not.  I have double
> embedded tissues before, in paraffin, control blocks are often done
> this way with cores of paraffin embedded tissues taken from blocks, all cores
> embedded in one block.
>
> Gayle Callis
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 00:14:01 -0600
> From: marg mcgee <mmcgee@medicine.adelaide.edu.au>
> Subject: Surface stains - ground sections
>
> Dear Histonetters
>
>
> I am keen to know what stains people are using at the moment for surface
> staining of ground sections of bone/implant specimens.
>
> I have used Tol blue in the past and have been very happy with it but was
> interested in using a red/blue stain.
>
> Thanks
>
> Marg McGee
>
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 00:16:14 -0600
> From: Cynthia Favara <cfavara@atlas.niaid.nih.gov>
> Subject: RE: To embed or not to embed?
>
> Amy,
> 	I would not recommend this but I can tell you that we have had lots of
> embedder problems and one time I had to  leave all the cassettes in a solid
> block for a week due to vacation and equipment malfunction. Everything came
> out fine, can't remember if ther were any immunos.
> Cynthia Favara
>
> > ----------
> > From: 	Woodfin, Amy C[SMTP:AWoodfin@peacehealth.org]
> > Sent: 	Monday, December 14, 1998 12:34 PM
> > To: 	'histonet@pathology.swmed.edu'
> > Subject: 	To embed or not to embed?
> >
> > Hi folks,
> >
> > Still on this wonderful RPI...no really it is!  The question has come up
> > whether it does any harm to the tissue to pull it out of the processor at
> > the end of the cycle and let them sit at room temperature for several hours
> > until they are embedded.  I can't find anything definitive in Carson,
> > Sheehan or AFIP.  Only references to crystalization of the paraffin.  There
> > is no mention if reheating the specimens several hours later and THEN
> > embedding them would have any ill effect.
> >
> > I know we've always done it as soon as the processor if finished, but is
> > that the only way?  Any input?
> >
> > Thanks!
> >
> > Amy Woodfin
> > St. Joseph Hospital - Pathology
> > Bellingham, WA
> > (360) 738-6340
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 00:16:38 -0600
> From: Cynthia Favara <cfavara@atlas.niaid.nih.gov>
> Subject: enhanced DAB
>
> Netters,
> 	Has anyone ever done any immunos using DAB with Imidzole or Osmium
>for a
> stronger[more definite] signal? Would like to do this in our lab and am
>in the
> dark. Thanks
>
> Cynthia Favara
> Rocky Mountain Laboratories
> 903 S 4th Street
> Hamilton, MT 59840
> ph: 406-363-9317
> FAX: 406-363-9286
> e-mail: cfavara@nih.gov
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 00:17:24 -0600
> From: "Shelton, John" <Shelton#m#_John@Ryburn.swmed.edu>
> Subject: Job Opening / Updated Web Site
>
>
> The Molecular Pathology Core Lab of the Ryburn Molecular Cardiology Center at
> UT Southwestern Medical Center is recruiting for registered histologists.
>The
> laboratory is presently seeking a Senior Histology Technician with precise
> microtomy skills and detailed knowledge of automatic tissue processing of
> research specimens.
>
> The Molecular Pathology Core provides morphologic analysis of gene expression
> research on-going at UT Southwestern, and collaborative institutions
> throughout the USA.  The laboratory primarily facilitates investigation of
> factors involved in heart and skeletal muscle development through
> characterization of transgenic mouse and gene knockout models; however,
> characterization of models in other organ systems are performed as well.
> Histology of the labs collaborative research endeavors have appeared on the
> covers of Cell (twice, this year alone), Genes and Development, Development,
> and many, many others.
>
> A bachelors degree in the life sciences and one year of experience as a
> registered HT is requested; experience in lieu of degree will be considered.
> Please forward resume, transcripts, and salary history to:
> UT Southwestern Medical Center
> 5323 Harry Hines Blvd.
> Dallas, Texas 75235-8573
> Attn:  John M. Shelton      or    shelton@ryburn.swmed.edu
>
> UT Southwestern is an equal opportunity employer
>
>________________________________________________________________________________
> Still searching for the right person...
> Drop by the website to see more:
> http://www.swmed.edu/home_pages/cardiology/labs/jarlab.html
>
>________________________________________________________________________________
>
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 00:17:50 -0600
> From: "Jeff Silverman" <peptolab@hamptons.com>
> Subject: Re: To embed or not to embed?
>
> Amy- Cooling and reheating is no problem for routine surgcial specimens- I
> do it all the time.
>
> Jeff Silverman
>
> - ----------
> > From: Woodfin, Amy C <AWoodfin@peacehealth.org>
> > To: 'histonet@pathology.swmed.edu'
> > Subject: To embed or not to embed?
> > Date: Monday, December 14, 1998 2:34 PM
> >
> > Hi folks,
> >
> > Still on this wonderful RPI...no really it is!  The question has come up
> > whether it does any harm to the tissue to pull it out of the processor at
> > the end of the cycle and let them sit at room temperature for several
> hours
> > until they are embedded.  I can't find anything definitive in Carson,
> > Sheehan or AFIP.  Only references to crystalization of the paraffin.
> There
> > is no mention if reheating the specimens several hours later and THEN
> > embedding them would have any ill effect.
> >
> > I know we've always done it as soon as the processor if finished, but is
> > that the only way?  Any input?
> >
> > Thanks!
> >
> > Amy Woodfin
> > St. Joseph Hospital - Pathology
> > Bellingham, WA
> > (360) 738-6340
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 00:18:23 -0600
> From: "Mark & Carrie Byrne" <eire@teleport.com>
> Subject: RE: To embed or not to embed?
>
> hi amy,
> i've done this, and not for just a few hours.  i've routinely "stored"
> control tissue in an infiltrated but not embedded condition with no
> detrimental effect to the procedure.  and the cassettes take less space to
> store than blocked tissue.
> happy holidays,
> carrie kyle-byrne
> eire@teleport.com
>
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 01:45:17 -0600
> From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
> Subject: Re: Wavy sections
>
> On Mon, 14 Dec 1998, Marilyn Woods wrote:
>
> > Looking for suggestions on the cause of wavy sections, so bad that we are
> > unable to focus under high power.  This has happen out of the blue without
> > changing any procedures.  I am cutting all skin, some look good,  but this
> > problem seems to affect the staining ex. muddy appearing, poor contrast and
> > understaining.  All temps. are with norm. except of a paraffin bath on
> > processor is 64 C. Sections have been good 2 days a week then progressively
> > get worse.
>
>    Your use of the word "wavy" suggests that the thickness varies,
>    in stripes or bands parallel to the knife edge, or that wrinkles
>    were not flattened out. The former is more likely because you
>    would have seen failure to get rid of wrinkles. The artifact of
>    thickness varying as the knife cuts through the block is called
>    "chatter." Perhaps this is what you have. It is attributed to
>    vibration of either the knife edge or the specimen, and the
>    remedy is to tighten everything up on the microtome, including
>    the little screws that hold a disposable blade onto its support
>    (if applicable to your equipment).
>
>    For an excellent account of chatter and many other cutting artifacts,
>    see "An Introduction to Histotechnology" by Geoffrey Brown (Appleton
>    Century Crofts, New York, 1978. ISBN 0-8385-4340-5). I don't know
>    if it's still in print. I found my copy quite recently, by chance,
>    in a second-hand bookshop, having been previously unaware of it.
>    This nice hard-cover volume may not have got enough publicity. The
>    practical instructions and hints about details are first rate. The
>    author evidently draws on experience in the U.K. and U.S.A.
>
>    This may not answer your question fully, because even uneven sections
>    and those with chatter can be brought into focus if the microscope
>    knob is continually adjusted while moving across the field. Maybe
>    more than one factor is involved, but something wrong in the cutting
>    is probable on the strength of what you say.
>
>  John A. Kiernan,
>  Department of Anatomy & Cell Biology,
>  The University of Western Ontario,
>  LONDON,  Canada  N6A 5C1
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 14:28:26 -0600
> From: Jim Elsam <jim@hteqa.demon.co.uk>
> Subject: eggwhite as block for IHC
>
> Dear Bonnie
>
> The article your pathologist is after is possibly:
>
> Miller RT, Kubier P. Blocking endogenous avidin-binding activity in
> immunohistochemistry. Appl immunohistochem 1997;5:63-66
>
> You might also have a look at:
>
> Clark P et al. Increased avidin binding due to antigen retrieval:
> strategies for indentifying and reducing this widespread phenomenon. J
> Cell Path 1998;3,105-112.
>
> Good luck!
> - --
> Jim Elsam
> HTEQA Services
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 14:29:29 -0600
> From: Greg Dobbin <dobbin@Upei.CA>
> Subject: Re: eggwhite as block for IHC
>
> Bonnie wrote:
> > Date:          Mon, 14 Dec 1998 09:46:09 -0600
> > From:          Bonnie Whitaker <bwhita@casper.med.uth.tmc.edu>
> > Subject:       eggwhite as block for IHC
> > To:            Histonet@Pathology.swmed.edu
>
> > Hi All,
> >
> > I hope everyone had a pleasant weekend!!  I am in need of help!  Can anyone
> > tell me where to find an article on the use of egg white as the source of
> > avidin for an avidin-biotin block?  My pathologist knows he has it
> > "somewhere" but neither of us have been able to locate it.
> > Thanks in advance!!
> >
> > Bonnie Whitaker
> > UT--Houston
> >
> The reference is:
>
> Miller RT, Kubier P: Blocking of endogenous avidin-binding activity
> in immunohistochemistry: The use of of egg whites.
> APPLIED IMMUNOHISTOCHEMISTRY 5(1): 63-66, 1997.
> >
> >
> >
> >
> >
> >
> Greg Dobbin
> Pathology Lab
> Atlantic Veterinary College, U.P.E.I.
> 550 Unviversity Ave.
> Charlottetown, P.E.I.
> C1A 4P3
> Phone: (902)566-0744
> Fax: (902)566-0851
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 14:30:06 -0600
> From: "Kellar, Eric" <kellarec@MSX.UPMC.EDU>
> Subject: RE: T. evansi
>
> Louise:
> These single cell pathogens sound as if they could rival any character from
> an Anne Rice novel. Trypanosoma evansi belongs to a group of flagellate
> protozoa of the class Zoomastigophorea:Salivaria. They have complex life
> cycles involving 2 hosts. These New World trophozoites live in the blood,
> esophagus, intestine and then the salivary glands of their insect
> vector-host (Tabanus) or horse fly. They proliferate as trypomastigotes in
> the salivary glands of the fly, then are mechanically transmitted by bite to
> the camel, elephant, horse, donkey or burro.
> The clinical course of the infection is characterized by an increase in
> numbers, followed by a sudden decrease in the population which is repeated
> over a number of cycles. The remission coincides with an increase in host
> protective antibody against specific surface expressed antigens , located in
> the surface coat or glycocalyx.  The cyclic nature appears to occur due to
> an almost endless change in the Varient Antigen Type (VAT). As a result, as
> the host appears to be winning the battle, the parasite changes its variant
> antigen coating and evades the host immune response allowing a resurgence of
> the infection.
> In Mexico and South America, T. evansi is also transmitted by the
> blood-sucking Desmodus rotundus or vampire bat, whom preen and beg each
> other to share the tablespoon of infected blood. The ancient Maya and Inca
> also had their suspicions of the feared and revered flying God, whom shared
> in their blood cult, attaching themselves to their livestock at night. Soon
> after the bite, the victim would salivate, wither and die.
> T. evansi in mammals causes "Surra" (heavy breathing sound), and translated
> as rotten in India. It is also distributed throughout the Far East, North
> Africa and Central and South America. Canines may also be infected by
> feeding on contaminated carcasses, the pathogen invades their buccal mucosa.
>
> Depending on the host, T. evansi can be detected and classified with
> serology and PCR.  Histologically, it is demonstrated in spleen, bone marrow
> and lymph node of their vertebrate hosts with a Giemsa (for parasites),
> Wright stain or a methyl green pyronin (MGP).
> On a lighter note...Happy Holidays!
>
> Eric Kellar
> Histology/Immunohistochemistry
> University of Pittsburgh Medical Center
>
>
> 	From:  Louise Burrell [SMTP:lburrell@pathbox.wustl.edu]
> 	Sent:  Monday, December 14, 1998 3:59 PM
> 	To:  HistoNet Server
> 	Cc:  Laurie.Reilly@jcu.edu.au
> 	Subject:  Re:Hi old friend!!!
>
> 	Dear Laurie:
> 	I am having trouble getting thru to you.  My last e-mail was really
> 	meant(sent) to/for someone else.  please rite back--bad news re: my
> uncle
> 	Paul.
> 	warmest regards for a lovely holiday season for you and your
> family!!!
> 	What is Trypanosomes evansi???
>
> 	Love,
>
> 	 Beezie
>
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 14:30:50 -0600
> From: rschoonh@sph.unc.edu
> Subject: Geoffrey Brown's book
>
> John,
>
> The last time I saw Geoff was at dinner one night at the NSH meeting in
> Crystal
> City quite a few years ago.  At that time his book was out of print and I
> believe
> he told me then that the publisher sent him the unsold books (could be wrong
> about that as it HAS been a while).  He did send me one (autographed) after
> the
> meeting and that has been the last I've seen or heard from him.
>
> I remember him as a true Gentleman and would assume that he has long since
> retired.
>
> - -- Begin original message --
>
> >    Your use of the word "wavy" suggests that the thickness varies,
> >    in stripes or bands parallel to the knife edge, or that wrinkles
> >    were not flattened out. The former is more likely because you
> >    would have seen failure to get rid of wrinkles. The artifact of
> >    thickness varying as the knife cuts through the block is called
> >    "chatter." Perhaps this is what you have. It is attributed to
> >    vibration of either the knife edge or the specimen, and the
> >    remedy is to tighten everything up on the microtome, including
> >    the little screws that hold a disposable blade onto its support
> >    (if applicable to your equipment).
> >
> >    For an excellent account of chatter and many other cutting artifacts,
> >    see "An Introduction to Histotechnology" by Geoffrey Brown (Appleton
> >    Century Crofts, New York, 1978. ISBN 0-8385-4340-5). I don't know
> >    if it's still in print. I found my copy quite recently, by chance,
> >    in a second-hand bookshop, having been previously unaware of it.
> >    This nice hard-cover volume may not have got enough publicity. The
> >    practical instructions and hints about details are first rate. The
> >    author evidently draws on experience in the U.K. and U.S.A.
> >
> >    This may not answer your question fully, because even uneven sections
> >    and those with chatter can be brought into focus if the microscope
> >    knob is continually adjusted while moving across the field. Maybe
> >    more than one factor is involved, but something wrong in the cutting
> >    is probable on the strength of what you say.
> >
> >  John A. Kiernan,
> >  Department of Anatomy & Cell Biology,
> >  The University of Western Ontario,
> >  LONDON,  Canada  N6A 5C1
>
> - -- End original message --
>
> regards,
> Bob
> Robert Schoonhoven
> Laboratory of Molecular Carcinogenesis and Mutagenesis
> Dept. of Environmental Sciences and Engineering
> University of North Carolina
> CB#7400
> Chapel Hill, NC 27599
> Phone
> office 919-966-6343
>    Lab 919-966-6140
>    Fax 919-966-6123
>
> **I'm willing to make the mistakes if someone else is willing to learn from
> them**
>
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 14:31:17 -0600
> From: rschoonh@sph.unc.edu
> Subject: Re: enhanced DAB
>
>
> Cynthia,
>
> While I now use Innovex's DAB Enhancer, I have in the past (5-6 years ago)
> used Osmium.  I don't
> reccomend it for safety reasons.  NiCl metods also work well (carefull it's a
> carcinogen) but
> I've opted for the commercial (pricy) route because of time constraints.  I
> might add that most
> of the antiboby suppliers sell various DAB enhancing solutions.
>
> If you still want to use Osmium I have the method somewhere ........ and if
> you'll send me your
> fax # I will send it to you (may take a while as I'm not sure where I filed
> it).
>
> - -- Begin original message --
> >
> > Netters,
> >       Has anyone ever done any immunos using DAB with Imidzole or
>Osmium for
> a stronger[more
> > definite] signal? Would like to do this in our lab and am in the dark.
> Thanks
> >
> > Cynthia Favara
> > Rocky Mountain Laboratories
> > 903 S 4th Street
> > Hamilton, MT 59840
> > ph: 406-363-9317
> > FAX: 406-363-9286
> > e-mail: cfavara@nih.gov
>
> - -- End original message --
>
> regards,
> Bob
> Robert Schoonhoven
> Laboratory of Molecular Carcinogenesis and Mutagenesis
> Dept. of Environmental Sciences and Engineering
> University of North Carolina
> CB#7400
> Chapel Hill, NC 27599
> Phone
> office 919-966-6343
>    Lab 919-966-6140
>    Fax 919-966-6123
>
> **I'm willing to make the mistakes if someone else is willing to learn from
> them**
>
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 14:31:48 -0600
> From: rkline@emindustries.com
> Subject: Re: Wavy sections
>
> Marilyn,
>
> Infiltrating at 64 degrees is a little high.  It may be causing your
> tissues to harden and actually be cooking them.  Which would account for
> the muddy appearance and the waves from hardness.
>
> Remedy:
>
> Make sure all screws are tight on your microtome.  Chatter is also caused
> by the knife holder beind loose or angle being off.
>
>  Lower the temp of the paraffin baths to melting point or the paraffin
> being used or a couple degrees above.  I used to keep mine at 59 degrees C.
>
> Rough cut and soak hard to cut blocks in icy water or a neat trick is to
> plop them in your waterbath for a couple of seconds ( no more than that or
> else blocks will start to melt) and back on ice.  The warm water will
> soften them.  Or EM Science makes a softening agent called Mollifex that
> works quicker at softeneing.  If you need info on it contact me directly.
>
> Rande Kline HT (ASCP)
> Technical Services
> EM Science
>
>
>
>
> Marilyn Woods <mwoods@execpc.com> on 12/14/98 05:40:58 PM
>
> To:   Histonet <histonet@Pathology.swmed.edu>
> cc:
> Subject:  Wavy sections
>
>
>
>
> Looking for suggestions on the cause of wavy sections, so bad that we are
> unable to focus under high power.  This has happen out of the blue without
> changing any procedures.  I am cutting all skin, some look good,  but this
> problem seems to affect the staining ex. muddy appearing, poor contrast and
> understaining.  All temps. are with norm. except of a paraffin bath on
> processor is 64 C. Sections have been good 2 days a week then progressively
> get worse.
>
> Thank you
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 14:32:57 -0600
> From: Lynn Gardner <gardnerl@horus.ophth.uiowa.edu>
> Subject: Re: Wavy sections
>
> Good morning. The following are some suggestions on things we looked at and
> changed when we were experiencing this problem.
>
> The first thing I would check is what is the melting point of your
> paraffin, should be written on the box or MSDS. Your temperature on your
> processor seems quite high usually the temp should be for a 56-58 degree C
> melting point the temp on the processor should be set at 57 degrees C. The
> other thing is how long you process your tissues in the paraffin, it should
> be no more than 2.5 hours total time.
>
> The second thing I would look at is how thick the tissue sections are on
> the days you are having problems. If  they seem thicker on those days you
> may want to gross them thinner they may not be getting proper infiltration
> with the processing chemicals. Also how long did to tissues fix is there a
> variation in the fixation times. If there are make sure to find out how
> long the "good" tissues fixed prior to processing and use that as a
> standard and show the physicians why you want to do that, show them a good
> slide and a bad slide and help them understand why the tissue will be
> delayed a bit.
>
> The third option is to look at the way the tissue is being cut if the skin
> edge is horizontal _______ to the knife try changing it to the skin edge is
> vertical to the knife  | or if it is the other way around try switching it.
> Sometimes it is the way we cut the tissue that causes that problem.
>
> And Finally it may have to do with a combination problem first due to the
> excessive heat in you processing paraffin and then not enough heat in your
> waterbath. We use a two waterbath technique that helps to float out tissue.
> We use a waterbath at room temperature to float our tissue out and pick it
> up on a slide. We then place that piece on a hot water bath usually between
> 54 and 56 degrees C. Going from the room temp to the hot helps the paraffin
> expand better thus eliminating the waves. We work with corneal tissue and
> since we have used this technique we rarely see waves.
>
> Unfortunately without seeing the slides and tissues I can only offer these
> suggestions. One or a combination of these suggestions should work for you.
> I wish you well in your hunt for an answer.
>
> Please let me know if I can be of further assistance.
>
> Sincerely,
> Lynn Gardner, HT(ASCP)
> Supervisory Research Assistant III
> FC Blodi Eye Pathology Laboratory
> The University of Iowa
> 233 MRC
> Iowa City, IA 52242-1182
> Phone: 319-335-7095
> Fax: 319-335-7193
>
>
>
> At 04:40 PM 12/14/98 -0600, you wrote:
> >Looking for suggestions on the cause of wavy sections, so bad that we are
> >unable to focus under high power.  This has happen out of the blue without
> >changing any procedures.  I am cutting all skin, some look good,  but this
> >problem seems to affect the staining ex. muddy appearing, poor contrast and
> >understaining.  All temps. are with norm. except of a paraffin bath on
> >processor is 64 C. Sections have been good 2 days a week then progressively
> >get worse.
> >
> >Thank you
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 14:33:40 -0600
> From: Kelly Dunlap <dunlap@naiadtech.com>
> Subject: Employment Opportunity
>
> Dynamic Employment Opportunity in Portland, Oregon.
>
> Naiad Technologies, Inc. is currently recruiting for a Product Manager
> with minimum 3-5 years experience in the histopathology market.  Naiad
> Technologies, Inc. develops and markets products for reducing the cost
> and handling of liquid hazardous waste in clinical laboratories.
>
> Specific responsibilities include but are not limited to the following:
>
> Gathering of market intelligence to support product development;
> understanding users' practices and needs including features, support,
> quality and timeliness; acting as coordinator for all aspects of product
>
> definition, development and implementation; managing products in the
> sales channel including positioning, pricing, merchandising,
> forecasting, inventories and training; identifying and quantifying new
> market opportunities; and coordinating activities among departments to
> achieve maximum sales and profit potential.
>
> This position reports directly to the Director of Marketing.  Candidates
>
> should possess at least a Bachelors of Science degree in a technical
> field.  MBA preferred.  Special consideration given to candidates with
> hands-on histopathology laboratory experience.  Compensation
> commensurate with experience.
>
> Naiad offers a competitive compensation and benefit package, including
> stock options, a 401(K) plan and a stimulating work environment.  Naiad
> is an equal employment opportunity employer.  For more information visit
>
> our web site at www.naiadtech.com.
>
> Interested applicants should send resume and salary history to Naiad
> Technologies, Attn: Human Resources, 2611 SW 3rd, Suite 200, Portland,
> Oregon, 97201, or fax to 503-241-0827.
>
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 14:34:06 -0600
> From: "Ian Barclay" <koira@clara.net>
> Subject: Future Job in Highlands of Scotland
>
> There will be a vacancy arising in the Pathology Department at Raigmore
> Hospital, Inverness, Scotland.
> This post is for a basic grade Medical Laboratory Scientific Officer
> (Histotechnologist) and enquiries are welcomed from qualified personnel.
> Interested parties are invited to contact Blair France or Phil Burgin on
> (44)     (0)1463 704265 or by e-mailing either of the above at:
> raigmore@wintermute.co.uk
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 14:34:39 -0600
> From: dave@biocare.net
> Subject: Drying slides for ER
>
>
> We have been drying slides for ER at 60 degrees versus 37 degrees and
> comparing them for ER staining (1D5 clone).  We are observed reduced
> staining when drying at 60 degrees.  The longer we dry at 60 degrees the
> less staining we observed.  We have concluded that drying overnight 37
> degrees is superior to 60 degrees drying for 1-2 hours.
>
> If we have had problems with the tissue staying on the slide, we would
> dry slides in a 60 degree oven for 3-4 hours. We observed less staining
> intensity at 3-4 hours versus 1 hour.  We had dried some slides
> overnight at 60 degrees. 70-80% of the staining was reduced.
>
> Has anyone ever observed this phenomena before?  Let's chat!
>
> David Tacha HTL (ASCP)
> Biocare Medical
>
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 16:38:13 -0600
> From: "Berger, Jennifer" <jberger@lrri.org>
> Subject: test
>
> Is the histonet having problems again? I haven't received anything all
> day?
> Jennifer
> Jennifer Berger
> Lovelace Respiratory Research Institute
> Albuquerque, NM
> jberger@lrri.org
>
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 16:39:02 -0600
> From: "Sarah Christo" <schristo@CVM.TAMU.EDU>
> Subject: Re: Wavy sections -Reply
>
> Dear Marilyn,
>   If chatter is not your problem, I have seen this
> happen when sections come loose from the slides
> during staining.
> Sarah, Texas A&M
>
>
> On Mon, 14 Dec 1998, Marilyn Woods wrote:
>
> > Looking for suggestions on the cause of wavy sections, so bad that we are
> > unable to focus under high power.  This has happen out of the blue without
> > changing any procedures.  I am cutting all skin, some look good,  but this
> > problem seems to affect the staining ex. muddy appearing, poor contrast and
> > understaining.  All temps. are with norm. except of a paraffin bath on
> > processor is 64 C. Sections have been good 2 days a week then progressively
> > get worse.
>
>    Your use of the word "wavy" suggests that the thickness varies,
>    in stripes or bands parallel to the knife edge, or that wrinkles
>    were not flattened out. The former is more likely because you
>    would have seen failure to get rid of wrinkles. The artifact of
>    thickness varying as the knife cuts through the block is called
>    "chatter." Perhaps this is what you have. It is attributed to
>    vibration of either the knife edge or the specimen, and the
>    remedy is to tighten everything up on the microtome, including
>    the little screws that hold a disposable blade onto its support
>    (if applicable to your equipment).
>
>    For an excellent account of chatter and many other cutting artifacts,
>    see "An Introduction to Histotechnology" by Geoffrey Brown (Appleton
>    Century Crofts, New York, 1978. ISBN 0-8385-4340-5). I don't know
>    if it's still in print. I found my copy quite recently, by chance,
>    in a second-hand bookshop, having been previously unaware of it.
>    This nice hard-cover volume may not have got enough publicity. The
>    practical instructions and hints about details are first rate. The
>    author evidently draws on experience in the U.K. and U.S.A.
>
>    This may not answer your question fully, because even uneven sections
>    and those with chatter can be brought into focus if the microscope
>    knob is continually adjusted while moving across the field. Maybe
>    more than one factor is involved, but something wrong in the cutting
>    is probable on the strength of what you say.
>
>  John A. Kiernan,
>  Department of Anatomy & Cell Biology,
>  The University of Western Ontario,
>  LONDON,  Canada  N6A 5C1
>
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 18:15:24 -0600
> From: Roger Gallant <roggal@dsuper.net>
> Subject: Please unsubscribe
>
> Please unsubscribe for the holidays!
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 19:00:38 -0600
> From: Rcia1@aol.com
> Subject: Re: Drying slides for ER
>
> We started drying our ER/PR slides in a 80 degree oven for 30 minutes.  We
> first got wind of this through the Ventana support group.  We've been doing
> antigen retrieval in a microwave pressure cooker, for no more than 30
>minutes,
> usually 10 mininute when up to pressure, which usually takes 10 minutes.
> Sometimes we have problems with sections falling off.   After all this,
>it's a
> wonder they stay on at all.
> Anyway, my guess, the problem of falling off, is due to the tissue, ie. too
> much fat, under fixation and any other excuse that takes the blame off of us
> fantastic histotechs.  Hope my two cents worth, helps.
>
>
> ----------------------------------------------------------------------
>
> Date: 15 Dec 1998 20:00:43 -0600
> From: "Mark & Carrie Byrne" <eire@teleport.com>
> Subject: RE: Drying slides for ER
>
> hi dave,
> i have also seen a decrease in aby "staining" intensity when left in a 60
> degree oven for more than 1 hour (on many different aby's...for this reason,
> i NEVER leave them in longer that that).
> however, i've not encountered a decrease in intensity vs. longer/cooler
> heating.  but then i've not used a 37 oven either.  the only comparison i've
> made is a weekend/room temp. vs 1hr/60 degree.  i was unable to tell the two
> apart (we also use the 1D5 clone).
> i'm assuming that your 60 oven is maintaining a very stable temp.
> interesting......
> carrie kyle-byrne
> eire@teleport.com
>
>
>
> Here are the messages received yesterday!
>
>