Alan,
	I have been gone and have missred this discussion. My question is do
you maintain the cryostat at -8C to -12C and then cool the knife?

Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286
e-mail: cfavara@nih.gov


> ----------
> From: 	Alan Bright[SMTP:Bright@dial.pipex.com]
> Sent: 	Tuesday, December 22, 1998 7:51 AM
> To: 	Histonet@pathology.swmed.edu
> Subject: 	Re: sectioning thick frozens
>
> I cannot understand why so many Histonetters are having problems and
> avoiding thick frozen sections ?
>
> The main points to follow to achieve thick frozen sections  are:
>
> 1) Correct tissue temperature is most important, to cold will cause
> excessive cracking.
>
> 2) Brain must be sectioned between -8 to -12deg.C. & the knife temperature
> should be around -10deg.C. colder than the tissue. By using this method
> sections are cut up to 300 microns routinely.
>
> Merry Christmas to All,
>
>
> Alan Bright
>
> Bright Instrument Co.Ltd.
> St Margarets Way
> Huntingdon
> PE18 6EB
> England
>
> Tel No; 01480 454528
> Fax No;01480 456031
> Email ; Bright@dial.pipex.com
>
>
>
>