RE: immunostain enhancement
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From: | "Hawkins, Hal" <hhawkins@SBI.UTMB.EDU> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Since we do our immunostaining in an EM lab, we often use
osmium to darken the reaction product. We simply put a
drop of our standard 1% cacodylate-buffered osmium tetroxide
on each section in a hood and rinse it off in running tap water
after about 2 seconds. It makes light DAB reaction product
pretty dark brown, and adds a light brownish tint to the rest
of the section. The hood is crucial, though -- without good
ventilation, you will start to notice rings around lights very quickly!
Hal Hawkins
UTMB Galveston
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