RE: Bosma Steiner stain
<< Previous Message | Next Message >>
From: | "Kellar, Eric" <kellarec@MSX.UPMC.EDU> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Dr. Tyler:
I have tried the modification of the Steiner microwave technique introduced
by Bosma in 1984*, and later by Elias and Bosma in 1987. These methods
employ the use of 'alpha-amylase' to predigest the sections. My preference
is the Steiner microwave modification by Garvey W. et al: Modified Steiner
for the demonstration of spirochetes. J. Histotechnology 8: 15-17, 1985. I
have also stained spirochetes with the classic and modified microwave
methods of Dieterle, Steiner and Steiner and Warthin-Starry. The choice of
methods appears to be a personal one. All the methods differ slightly with
the use of uranyl nitrate as a sensitizer, pH, reproducibility and turn
around time.
I have all of the above mentioned methods and would be happy to fax you a
copy.
*Boon ME, Kok LP (1988) Microwave Cookbook of Pathology: The Art of
Microscopic Visualization. Leiden: Coulomb Press Leyden.
Eric Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center
----------
From: wbtyler@webtv.net [SMTP:wbtyler@webtv.net]
Sent: Saturday, December 19, 1998 1:32 AM
To: HistoNet@Pathology.swmed.edu
Subject: Bosma Steiner stain
Does anyone have a reference for the procedure for this modification
of
the Steiner stain? Is anyone using this procedure?
Thank you.
William B. Tyler MD
Department of Pathology
Geisinger Medical Center
Danville, PA 17821
Penn State Geisinger Health System
Email:wtyler@psghs.edu
<< Previous Message | Next Message >>