RE: ANTIGEN RETRIEVAL

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From:"Sebree Linda A." <la.sebree@hosp.wisc.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Mary,

Try 1mM EDTA, pH 8 for anywhere from 5-40" in some type of heating
apparatus, i.e. water bath, microwave oven, hot plate, pressure cooker, etc.
We bring our slides and buffer up to just boiling, then incubate them in a
hot (91-94 degrees C) water bath for the duration.  We used to cool under
running tap water for 20" before staining but recently we've tried going
right from retrieval to staining with no discernible differences; sure saves
time!

Linda

Linda Sebree, HT
University of Wisconsin Hospital & Clinics
Department of Laboratory Medicine
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI  53792-2472

Phone:  (608)265-6596
FAX:  (608)263-1568

> -----Original Message-----
> From:	Mary L. Verlinde [SMTP:verlinde@ahdlms.cvm.msu.edu]
> Sent:	Thursday, December 17, 1998 1:07 PM
> To:	'HISTONET@PATHOLOGY.SWMED.EDU'
> Subject:	ANTIGEN RETRIEVAL
>
>
> I am looking for a recipe for an antigen retrieval acid or buffer for
> paraffin slides to do IHC. Thanks in advance.
>
>
>
> Mary Verlinde
> Animal Health Diagnostic Lab
> MSU




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