Desmin on BM BXs

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From:"Sebree Linda A." <> (by way of histonet)
To:histonet <>
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Hi Histonetters and Happy Holidays,

We're having problems getting desmin to work on B5 fixed BM BXs.  We've been
using a bx that is full of tumor cells metastasized from a strongly desmin
positive primary.  We routinely use Neomarkers antibody, clone D33, at a
1:30 dilultion after 20" EDTA HIER on formalin fixed tissue.  So far,
everything I've tried (combinations of heat retrieval and enzyme treatment
as well as each alone) has resulted in negative results.  Any help out


Linda Sebree, HT
University of Wisconsin Hospital & Clinics
Department of Laboratory Medicine
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI  53792-2472

Phone:  (608)265-6596
FAX:  (608)263-1568

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