Autofluorescence
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From: | "Michael J. Lyon, Ph.D." <lyonm@VAX.CS.HSCSYR.EDU> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hello:
I have just started doing immunostaining of muscle dissections. The tissue
has been immersion fixed in 4% paraformaldehyde 0.2% Picric acid for about
24 hrs at 4C. My primary antibodies are all fairly dilute at 1:3000 or
1:4000, secondary is at 1:400 and I am using CY3 at 1:600. I have tried
ammonium chloride and sodium borohydride but still have considerable
background from the muscle fibers. I was thinking of switching to a
different fixation. Any suggestions would be appreciated. Hope I have
given sufficient info.
Thanks in advance
Michael
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