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From:Masayuki Miyagishima <> (by way of histonet)
To:histonet <>
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I think the issue of saponin permeabilisation was once discussed here,
but let me ask a question, again.

When you used saponin, do you always need to use the saponin/PBS for
each wash?  Can saponin interfare with hydogen peroxide, antibodies, DAB
substrate, or any fluorescence?
I know some people just use for the first 30 min. permeabilisation step
for TUNEL.  Are there any reason for that?

Thank you for your help in advance.
Masayuki Miyagishima, MD
University of Pittsburgh,
Department of Surgery

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