Richard,

We use Direct Red 80 from Sigma Chem. Co. (Cat# D 030) in our Picrosirius
Red staining method for collagen.  You view your PS Red stained slides under
cross-polarized light to view the birefringent collagen.  This can be
quantitated using image analysis.  The PS Red method stains mostly collagen
types I, II, and III.

PS Red Staining solution:

0.25 g Direct Red 80 (Sirius Red F3B)
in
250 ml Saturated Picric Acid (Sigma Chem. Co)

mix and filter

We follow the staining method by Junquera et al.

References:
L.C. Junquera et al. 1986. Differential histologic diagnosis of osteoid:A
study on the human osteosarcoma collagen by the histochemical
picrosirius-polarization method. Journal of Pathology, 148:189-196.

R.G. Krafty, J Ivey, and M.R. Roach 1996.  Collagen Quantification by
Video-Microdensitometry in Rabbit Atherosclerosis. Matrix Biology,
15:141-144.

Philip Seifert
Harvard-MIT Division of Health Sciences and Technology
Biomedical Engineering Center
77 Massachusetts Avenue
Cambridge, MA 02139


> Date: Wed, 09 Dec 1998 10:17:34 +1300
> From: Ken Turner <ken.turner@stonebow.otago.ac.nz>
> Subject: re: Picro-serius red
> To: Richard Geering <sbs98rg@wye.ac.uk>
> Cc: HistoNet@Pathology.swmed.edu
> Mime-Version: 1.0
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Richard
>
> We have used sirius red F3B as an alternative to acid fuchsin in our Van
> Gieson (picro fuchsin) stain as its light fastness is better. Our mixture
> is as follows:
>         Sirius red F3B    .....250mg
>         Conc. Nitric acid .....0.5cc
>         Glycerol          .....10cc
>         Distilled water   .....90cc
>         Picric acid       .....to saturation
>
> We stain for 1 - 5 min depending on tissue and depth of stain required. We
> usually pre-stain nuclei for 5 - 10 min with Weigerts haematoxylin. Hope
> this is of some use to you.
>
> Sirius red F3B is available from BDH/Gurr cat.no. 341492F.
>
> Cheers
>
> Ken
>
>
> >Histonet collegues,
> >Thank you for adding me to the subscribers list. I am an equine
> >clinician now teaching. I am particularly interested in looking at
> >collagen in the equine lung and it has been suggeseted that I might
> >use a stain that I beleive is called Picro serius red. Is that
> >appropriate? Has any one the protocol and a supplier etc?
> >Thanking you in anticipation,
> >*********************************
> >Richard R Geering
> >Equine Science
> >Department of Biological Sciences
> >Wye College
> >Wye
> >Ashford
> >Kent TN25 5AH
> >*********************************
> >
> >Tel: +44 (0)1233 812401 ext 466
> >Fax: +44 (0)1233 813140
> >E-mail: r.geering@wye.ac.uk
>
>
> Kenneth W Turner
> Senior Teaching Fellow
> Unit Manager / Pathology Histology Unit
> Department of Pathology
> Otago Medical School
> P.O.Box 913
> Dunedin
> New Zealand
>
> Tel. 03 4797135  International  0064 3 4797135
> Fax. 03 4797136  International  0064 3 4797136
>
>
>