heat antigen retrieval

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From:Masayuki Miyagishima <mmiyagis+@pitt.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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I was suggested to do HEAT ANTIGEN RETRIEVAL for frozen tissue sample.

Does it work for frozen tissue, too?  Do I have to do some modification
on protocol?  I am planning to cover slides with 10 mM sodium citrate
buffer, pH 6.0 and put into micorwave for 10 min.
--
Masayuki Miyagishima, MD
University of Pittsburgh,
Department of Surgery
412-647-2345, and ask the hospital operator to page 3228, please.




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