fixation for in-situ PCR
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From: | Cathy Mayton <histology@the-onramp.net> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Fellow histonetters,
I am asking this question for a colleague of mine in SLC. The goal is to
paraffin embed monkey brain and kidney for in-situ PCR and in-situ RT-PCR.
They mentioned that other labs are using 2-4% paraformaldehyde for
fixation. Is this the correct percent and what would the fixation time be
for specimens approx 1 cm square?
Thank you in advance,
Cathy
******************************
Cathy A. Mayton
Wasatch Histo Consultants, Inc.
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