embedding in paraffin after RT

<< Previous Message | Next Message >>
From:Gayle Callis <uvsgc@msu.oscs.montana.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
Content-Type:text/plain; charset="us-ascii"

Had a friend who did just that in a teaching situation,  without any problems
with cutting.  I was a bit surprised that they embedded the infiltrated
tissues after they had allowed the paraffin to harden, but it seemed to
work.  I think one should look out for bubbles forming around the cooler
infiltrated tissue, but if one was careful, why not.  I have double
embedded tissues before, in paraffin, control blocks are often done
this way with cores of paraffin embedded tissues taken from blocks, all cores
embedded in one block.

Gayle Callis

<< Previous Message | Next Message >>