bone frozen sections/snap freeze/PVA
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| From: | Gayle Callis <uvsgc@msu.oscs.montana.edu> (by way of histonet) |
| To: | histonet <histonet@magicnet.net> |
| Reply-To: | |
| Content-Type: | text/plain; charset="us-ascii" |
I need to make a correction on the polyvinyl alcohol I recommended for
coating bone specimens before immersion in the dry ice/hexane or
isopentane slurry.
the polyvinyl alcohol (Sigma P-8136) should have a molecular weight of
30,000 - 70,000) and make a 4-5% solution in water.
Higher molecular weight PVA will not go into solution, tried it the other
day and realized I gave the wrong MW PVA info. Sorry for any inconvenience.
Gayle Callis
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