Vacuum pressure/hand processing

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We use a very old vacuum oven to manually process tissue cassettes for any
cases thought to be incompatible with the automatic tissue processor loads
(primarily our suspected CJD cases).  We keep the temperature constant at 60C,
and the pressure between 12-15 psi.  It has been our experience that when you
use any higher pressure readings, tissue morphology is adversely affected.
Our standard times for brain blocks were 45 mins per reagent change; biopsies
are run at 15 mins.
One word to the wise--don't screw or snap any lids onto the specimen cups...

Sue Lewis, BS  HTL(ASCP)

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