Re: wet tissue storage
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From: | rschoonh@sph.unc.edu (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Patsy,
I've done this several times (for some large studies) and stored the tissues
in 70% ETOH at 4oC and have not had any problems with our IHC. Granted the
4oC storage may have been overkill but it made ME feel better.
-- Begin original message --
>
> For you molecular chemists:
> If you have carefully aldehyde fixed (not too long for IHC) and then
> carefully decalcified (checking the end point, not over decalcifing) but
> cannot process the tissues for a few days, what should they be stored in
> for best results for IHC.
> Patsy
-- End original message --
regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone
office 919-966-6343
Lab 919-966-6140
Fax 919-966-6123
**I'm willing to make the mistakes if someone else is willing to learn from
them**
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