Re: storage of antigen retrieved slides

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From:Mick Rentsch <> (by way of histonet)
To:histonet <>
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O&P's certainly will become negative if left too long after heat retreival,
and this may be as little as 30 mins. Presumably the retreival results in
the exposure of sites to reaction by eg. encoiling of molecules or altering
the steric forces in the area, and these changes are reversible so long as
the tissue is not coagulated or cooked to a crisp.
Regards Mike Rentsch (Downunder)
-----Original Message-----
From: Mark & Carrie Byrne <>
To: Frank Walmsley <frankw@U.Arizona.EDU>; histonet
Date: Friday, 4 December 1998 1:05
Subject: RE: storage of antigen retrieved slides

>it has been my experience (and i know i've read it somewhere before, just
>can't remember where....darn formalin!) that you can't AR slides and them
>hold them for later IHC.  the IHC protocol should be started promptly after
>cooling.  i'd guess the all the cross-linking you just undid with the AR
>reforms.  anyone else out there know the detailed reasons?
>you'll probably need to do your AR again when you're ready to "stain" or
>just recut the slides and start over.
>carrie kyle-byrne

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