Re: pressure/hand processing/vacuum oven
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From: | "Ford M. Royer" <froyer@bitstream.net> (by way of Histonet) (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
I know that this is REALLY nit-picking, so please forgive me. The
operative word here is "mimic vacuum/ pressure". To truly duplicate the
alternating pressure/ vacuum cycle (PVC) of the automated processors that
have this feature, you would have to add pressure (0.3 - 0.35 Kg/cm2 )
after you have released the vacuum. The cycle on the automated processors
goes: Pressure ... Ambient ... Vacuum ... Ambient, repeatedly. However
this is not to say that the procedure described would not work just as
well. As I said, ..."nit-picking". :)
~ Ford Royer
Gayle Callis (by way of Histonet) wrote:
> We draw a vacuum to 15 - 20 psi, make sure the thermometer is visible
> with temp set at 60C or so. You can mimic a vacuum pressure automated
> processor by pulling the vacuum, releasing it after a specified time
> then draw the vacuum again. Voila! Alternating vacuum pressure,
> with good infiltration! This can be improve dense tissue infiltration.
>
> Gayle Callis
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