Re: pressure/hand processing/vacuum oven

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From:"Ford M. Royer" <> (by way of Histonet) (by way of histonet)
To:histonet <>
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I know that this is REALLY nit-picking, so please forgive me.  The
operative word here is "mimic vacuum/ pressure".  To truly duplicate the
alternating pressure/ vacuum cycle (PVC) of the automated processors that
have this feature, you would have to add pressure (0.3 - 0.35 Kg/cm2 )
after you have released the vacuum.  The cycle on the automated processors
goes: Pressure ... Ambient ... Vacuum ... Ambient, repeatedly.  However
this is not to say that the procedure described would not work just as
well.  As I said, ..."nit-picking".   :)

~ Ford Royer

Gayle Callis (by way of Histonet) wrote:

> We draw a vacuum to 15 - 20 psi, make sure the thermometer is visible
> with temp set at 60C or so. You can mimic a vacuum pressure automated
> processor by pulling the vacuum, releasing it after a specified time
> then draw the vacuum again.  Voila!  Alternating vacuum pressure,
> with good infiltration!  This can be improve dense tissue infiltration.
> Gayle Callis

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