Re: iodine from Betadine (histochem etc)
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
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On Wed, 9 Dec 1998 PSMannan@aol.com wrote:
> I am trying to find a method to detect iodine (from betadine) by
> histochemical methods. Any suggestions?
IF Betadine is the same as "povidone iodine" (stuff used to
disinfect hands etc) it's a combination of iodine with
polyvinylpyrollidone (PVP), from which the iodine is slowly
released so that it will do its stuff on the bacteria for
longer than a plain iodine solution. (The deposited iodine
sublimes, and the stained skin unstains in a few hours without
having to be washed.) This is derived from memories of the
mid-1960s in the Casualty Department at what was then the East
Birmingham Hospital. That's the B'ham or Brum where I was brung
up, what competed with Glasgow for being Britain's 2nd most
populous city. (Anyone know the current score? Ian M ?)
To the point. There's not much chance of the elemental iodine
staying in place for histochemical demonstration. It might bind
covalently to double bonds of lipids and/or the phenolic ring of
tyrosine in proteins. I've no idea how it could be made visible
if it does.
HOWEVER, the PVP component is a macromolecule and might well
be detectable. There is a staining method for PVP using Congo
red; it may not be the only one. See Pearse's Histochemistry
for rationale and instructions. PVP was used as a plasma
substitute. Perhaps the less successful uses or "complete
cases" led to the need for a method to show where it ended up.
PVP is also a very good as an aqueous mounting medium; another
of Pearse's achievements, I think. It's cheap, dissolves quite
quickly, and tolerates such additives as buffers and anti-fade
compounds. This last paragraph is just a little plug for the
polymer. Nothing to do with iodine!
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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