Re: formalin

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From:Laurence Reilly <> (by way of histonet)
To:histonet <>
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Back in the 60s we used Phosphate buffered formalin according to the recipe
in Drury and Wallington's version of "Carleton's Histological Technique",
which was a new book then. We still use the same formula.

                Regards,   Laurie.

At 10:27 AM 12/9/1998 +0000, you wrote:
>Hello histonetters,
>I am currently engaged on a seriously retrospective project involving
>performing PCR on  paraffin embedded archival tissues some of which
>are more than 30 years old. The variablity of the DNA viability and
>content is astounding. A factor that I can only ascribe to the
>various fixatives used way back then.
>Could some of the older histotechnologists give me some insight into
>what was common practice in say, the late 50's early 60's?
>(I will understand if some of you just say that you are reporting
>hearsay - after all - we don't all want to reveal our age?)
>When did buffered formalin come into vogue? As a mere whippersnapper
>of some 17 years experience - I can recall the unpleasantness of
>trying to make up buffered formalin in 25l quantities - an innovation
>and a great pain to a raw recruit.!
>I will appreciate any insight into what really happened
>many thanks
>Louise Taylor
>South Sfrica

Mr.Laurie Reilly.                      Telephone  07 4781 4468
Senior Technical Officer,              Facsimile  07 4781 5558
Physiology and Pharmacology,
Australian Institute of Tropical Veterinary and Animal Science,
James Cook University,
Townsville,  4811

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