Cynthia,

While I now use Innovex's DAB Enhancer, I have in the past (5-6 years ago)
used Osmium.  I don't
reccomend it for safety reasons.  NiCl metods also work well (carefull it's
a carcinogen) but
I've opted for the commercial (pricy) route because of time constraints.  I
might add that most
of the antiboby suppliers sell various DAB enhancing solutions.

If you still want to use Osmium I have the method somewhere ........ and if
you'll send me your
fax # I will send it to you (may take a while as I'm not sure where I filed
it).

-- Begin original message --
>
> Netters,
>       Has anyone ever done any immunos using DAB with Imidzole or Osmium
>for a stronger[more
> definite] signal? Would like to do this in our lab and am in the dark. Thanks
>
> Cynthia Favara
> Rocky Mountain Laboratories
> 903 S 4th Street
> Hamilton, MT 59840
> ph: 406-363-9317
> FAX: 406-363-9286
> e-mail: cfavara@nih.gov

-- End original message --

regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123

**I'm willing to make the mistakes if someone else is willing to learn from
them**