Re: eggwhite as block for IHC

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From:Greg Dobbin <dobbin@Upei.CA> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Bonnie wrote:
> Date:          Mon, 14 Dec 1998 09:46:09 -0600
> From:          Bonnie Whitaker <bwhita@casper.med.uth.tmc.edu>
> Subject:       eggwhite as block for IHC
> To:            Histonet@Pathology.swmed.edu

> Hi All,
>
> I hope everyone had a pleasant weekend!!  I am in need of help!  Can anyone
> tell me where to find an article on the use of egg white as the source of
> avidin for an avidin-biotin block?  My pathologist knows he has it
> "somewhere" but neither of us have been able to locate it.
> Thanks in advance!!
>
> Bonnie Whitaker
> UT--Houston
>
The reference is:

Miller RT, Kubier P: Blocking of endogenous avidin-binding activity
in immunohistochemistry: The use of of egg whites.
APPLIED IMMUNOHISTOCHEMISTRY 5(1): 63-66, 1997.
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Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 Unviversity Ave.
Charlottetown, P.E.I.
C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851




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