Re: control slide drying

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From:Mick Rentsch <> (by way of histonet)
To:histonet <>
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 One would think it wouldn't matter. My own personal preference is for a
separate control slide and these are invariably cut at the same thickness,
and all slides whether control or not are dried for 15-20 mins in a home
made fan forced drier @45C, no matter what the slide is for or if patient or
I might add though, that we have always elected to have the control on a
separate slide, particularly as more often than not we are are performing
these "specials" on more than one patient, as the local surgeons tend to do
like procedures on the same day and it's not uncommon to do 3 or 4 O&P's on
as many mastectomies or breast biopsies. Larger services would do many many
Our control slides are not filed with the patient as we found this to be
impractical; instead we maintain separate control files arranged in date
order, and a log book which records not only those patients to which the
control belongs, but also the grading of reaction etc. so that any
deterioration in performance is detected at an early stage either in or both
the antigenicity of the control slide or the efficiacy of reagents or
Regards Mike (Downunder)
-----Original Message-----
From: <>
To: <>
Date: Sunday, 13 December 1998 6:33
Subject: control slide drying

>   First let me explain, we are the result of many hospital/lab merges, so,
>have a long history and a very short memory, (long term exposure).  We were
>discussing the fact that some of us dry our control slides, after we cut
>and then box them up for later use and some of us cut the control slide,
>them air dry and then box them up for later use.  This is for IHC and
>stains.  We all put our patient on,  the same slide with the control  and
>them before we rehydrate.  Does it matter either way?
>Thanks for any input.
>Deb C.
>Metro Lab
>Davenport, IA.

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